A unique neuroendocrine cell model derived from the human foetal neural crest
| Autor: | Erica Sarchielli, Cristiana Deledda, Michaela Luconi, Serena Martinelli, Mario Maggi, M Pantaleo, Massimo Mannelli, Benedetta Fibbi, Elena Rapizzi, G. Marroncini, Gabriella B. Vannelli, Alessandro Peri, Susanna Benvenuti, Paola Luciani, Benedetta Mazzanti |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Homeobox protein NANOG Endocrinology Diabetes and Metabolism Primary Cell Culture SOX10 Cell 030209 endocrinology & metabolism Cell Separation Biology Cell Line 03 medical and health sciences Fetus 0302 clinical medicine Endocrinology Neuroendocrine Cells Cell Movement Pregnancy medicine Humans Neuroendocrine cell Neural crest Cell Differentiation Nestin Phenotype Cell biology ASCL1 030104 developmental biology medicine.anatomical_structure Neural Crest embryonic structures Female |
| Zdroj: | Journal of Endocrinological Investigation. 43:1259-1269 |
| ISSN: | 1720-8386 |
| Popis: | Nowadays, no human neuroendocrine cell models derived from the neural crest are available. In this study, we present non-transformed long-term primary Neural Crest Cells (NCCs) isolated from the trunk region of the neural crest at VIII–XII gestational weeks of human foetuses obtained from voluntary legal abortion. In NCC, quantitative real-time RT PCR demonstrated the expression of neural crest specifier genes, such as Snail1, Snail2/SLUG, Sox10, FoxD3, c-Myc, and p75NTR. Moreover, these cell populations expressed stemness markers (such as Nanog and nestin), as well as markers of motility and invasion (TAGLN, MMP9, CXCR4, and CXCR7), and of neuronal/glial differentiation (MAP2, GFAP, SYP, and TAU). Functional analysis demonstrated that these cells not only possessed high migration properties, but most importantly, they expressed markers of sympatho-adrenal lineage, such as ASCL1 and tyrosine hydroxylase (TH). Moreover, the expression of TH increased after the induction with two different protocols of differentiation towards neuronal and sympatho-adrenal phenotypes. Finally, exposure to conditioned culture media from NCC induced a mature phenotype in a neuronal cell model (namely SH-SY5Y), suggesting that NCC may also act like Schwann precursors. This unique human cell model provides a solid tool for future studies addressing the bases of human neural crest-derived neuroendocrine tumours. |
| Databáze: | OpenAIRE |
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