Correlation of the Blood Test Results Obtained between Assays Using Microliter-scale Fingertip Blood Samples Collected with a Novel Blood Collection Device and Conventional Venous Blood Assays: a Secondary Publication in English
| Autor: | Tomoaki Nishimura, Hajime Iwasawa, Kiyoaki Watanabe, Naoki Aikawa, Shota Nemoto |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Blood Glucose Male 0301 basic medicine Pathology medicine.medical_specialty medicine.drug_class Fingers 03 medical and health sciences Reference Values Humans Medicine Blood test Aspartate Aminotransferases Blood testing Physiological saline Volunteer Triglycerides Aged Glycated Hemoglobin Blood Specimen Collection 030109 nutrition & dietetics Chromatography medicine.diagnostic_test business.industry Cholesterol HDL Anticoagulant Cholesterol LDL gamma-Glutamyltransferase General Medicine Venous blood Blood collection Middle Aged Hematological test Healthy Volunteers Blood Cell Count Hematocrit Female business |
| Zdroj: | The Keio Journal of Medicine. 67:26-34 |
| ISSN: | 1880-1293 0022-9717 |
| DOI: | 10.2302/kjm.2017-0009-oa |
| Popis: | In this study, we investigated the correlations between biochemical and hematological test results obtained using microliter-scale fingertip blood samples collected with a newly developed blood collection device and those obtained using conventional venous blood. Eighty volunteer subjects were enrolled in this study. Blood samples were drawn from the fingertip of the ring finger by a single puncture, and 60-µL samples were promptly and accurately aspirated into a blood collection chip. Then the chip was tightly sealed in a chip container and was shaken to mix the contents without dispersion. For biochemical tests other than that for HbA1c, blood was collected without anticoagulant and centrifuged to obtain 15 µL of serum which was then diluted with 190 µL of physiological saline for the assay. For hematological tests and the test for HbA1c, the sample was assayed with blood collected using EDTA-2 K. Good correlations were obtained between the test results of the assay using fingertip blood and that using venous blood. The correlation coefficients were ≥0.97 for TG, T-CHO, HDL-C, LDL-C, GLU, ALT, γ-GTP, UA, BUN, and HbA1c and ≥0.95 for WBC, RBC, Hgb, and Hct. These results suggest that our microliter-scale blood testing system is comparable to assays using venous blood and may be useful as a rapid and simple test to determine basic clinical parameters that are close to the reference intervals. |
| Databáze: | OpenAIRE |
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