Adaptive optics plug-and-play setup for high-resolution microscopes with multi-actuator adaptive lens

Autor: Quintavalla, M., Pozzi, P., Verhaegen, M.H.G., Bijlsma, H.K., Verstraete, H.R.G.W., Bonora, S., Periasamy, A., So, P.T.C., König, K., Xie, X.S.
Rok vydání: 2018
Předmět:
Zdroj: Proceedings of SPIE: Multiphoton Microscopy in the Biomedical Sciences XVIII, 10498
DOI: 10.1117/12.2290036
Popis: Adaptive Optics (AO) has revealed as a very promising technique for high-resolution microscopy, where the presence of optical aberrations can easily compromise the image quality. Typical AO systems however, are almost impossible to implement on commercial microscopes. We propose a simple approach by using a Multi-actuator Adaptive Lens (MAL) that can be inserted right after the objective and works in conjunction with an image optimization software allowing for a wavefront sensorless correction. We presented the results obtained on several commercial microscopes among which a confocal microscope, a fluorescence microscope, a light sheet microscope and a multiphoton microscope.
Databáze: OpenAIRE