Tn5-mediated transposition of plasmid DNA after transduction to Myxococcus xanthus
| Autor: | John Downard |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
DNA
Bacterial Transposable element Microbiology chemistry.chemical_compound Plasmid Transduction Genetic Myxococcales Insertion sequence Myxococcus xanthus Molecular Biology Recombination Genetic Plasmid preparation Genetics biology Circular bacterial chromosome fungi Chromosomes Bacterial biology.organism_classification Molecular biology chemistry DNA Transposable Elements bacteria Homologous recombination DNA Research Article Plasmids |
| Zdroj: | Journal of Bacteriology. 170:4939-4941 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.170.10.4939-4941.1988 |
| Popis: | After coliphage P1-mediated transfer of Tn5-containing plasmid DNA from Escherichia coli to Myxococcus xanthus, transductants were identified which contained plasmid sequences integrated at many sites on the bacterial chromosome. The unaltered plasmid DNA sequences in these transductants were apparently flanked by intact Tn5 or IS50 sequences. These results suggest that Tn5-mediated transposition has occurred and provide a method for integrating plasmid DNA into the M. xanthus chromosome without the requirement for homologous recombination. |
| Databáze: | OpenAIRE |
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