The inhibitory role of Mir-29 in growth of breast cancer cells
| Autor: | Xiaona Huang, Xing Huang, Yujiang Guo, Qiang Zou, Zhenglong Wu |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Cancer Research
Breast cancer growth Down-Regulation Breast Neoplasms Cell Cycle Proteins Apoptosis Cancer progression Cell Growth Processes Biology Transfection medicine.disease_cause Breast cancer Cell Line Tumor microRNA Biomarkers Tumor medicine Humans Transcription factor Gene knockdown Base Sequence Research Genetic Therapy medicine.disease MicroRNAs Cell Transformation Neoplastic Oncology Gene Knockdown Techniques Immunology MCF-7 Cells Trans-Activators Cancer research Female Carcinogenesis Cyclin A2 Mir-29 |
| Zdroj: | Journal of Experimental & Clinical Cancer Research : CR |
| ISSN: | 1756-9966 |
| DOI: | 10.1186/1756-9966-32-98 |
| Popis: | Mir-29 microRNA families are involved in regulation of various types of cancers. Although Mir-29 was shown to play an inhibitory role in tumorigenesis, the role of Mir-29 in breast cancer still remains obscure. In this study, we showed that Mir-29a is the dominant isoform in its family in mammary cells and expression of Mir-29a was down-regulated in different types of breast cancers. Furthermore, over-expression of Mir-29a resulted in significant slower growth of breast cancer cells and caused higher percentage of cells at G0/G1 phase. Consistent with this over-expression data, knockdown of Mir-29a in normal mammary cells lead to higher cell growth rate, and higher percentage of cells entering S phase. We further found that Mir-29a negatively regulated expression of B-Myb, which is a transcription factor associated with tumorigenesis. The protein levels of Cyclin A2 and D1 are consistent with the protein level of B-Myb. Taken together, our data suggests Mir-29a plays an important role in inhibiting growth of breast cancer cells and arresting cells at G0/G1 phase. Our data also suggests that Mir-29a may suppress tumor growth through down-regulating B-Myb. |
| Databáze: | OpenAIRE |
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