Evaluation of respiratory model employing conventional NIH mice to access the immunity induced by cellular and acellular pertussis vaccines
| Autor: | Lucia Maria Correa Werneck, Ana Luíza Mattos-Guaraldi, Alexandre Alves de Souza de Oliveira Dias, Maria Aparecida Affonso Boller, Raphael Hirata Junior |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
Male Bordetella pertussis lcsh:Arctic medicine. Tropical medicine Time Factors lcsh:RC955-962 Whooping Cough lcsh:QR1-502 intranasal challenge model Acellular pertussis vaccines lcsh:Microbiology Mice Vaccines Acellular Immunity vaccine NIH conventional mice Medicine Animals Respiratory system Whooping cough Administration Intranasal Pertussis Vaccine Immunity Cellular Vaccines Synthetic biology business.industry pertussis Reproducibility of Results Gold standard (test) biology.organism_classification medicine.disease Virology Disease Models Animal Immunology Pertussis vaccine Nasal administration Female business medicine.drug |
| Zdroj: | Memórias do Instituto Oswaldo Cruz, Volume: 101, Issue: 7, Pages: 749-754, Published: NOV 2006 Memórias do Instituto Oswaldo Cruz., Vol 101, Iss 7, Pp 749-754 (2006) |
| ISSN: | 0074-0276 |
| Popis: | The increasing number of pertussis cases reported on the last twenty years and the existence of new acellular vaccines reinforce the need of research for experimental models to assure the quality of available pertussis vaccines. In this study, allotments of whole-cell and acellular pertussis vaccines were tested through the Intranasal Challenge Model (INM) using conventional NIH mice. The results have been compared to those achieved by the "Gold standard" Intracerebral Challenge Model (ICM). In contrast to ICM, INM results did not show intralaboratorial variations. Statistical analysis by Anova and Ancova tests revealed that the INM presented reproducibility and allowed identification and separation of different products, including three-component and four-component accellular pertussis vaccines. INM revealed differences between pertussis vaccines. INM provides lower distress to the mice allowing the reduction of mice number including the possibility of using conventional mice (less expensive) under non-aseptic environment. Thus, INM may be used as an alternative method of verifying the consistence of allotment production, including acellular pertussis vaccines. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|