Effects of Subchronic Exposure of Diclofenac on Growth, Histopathological Changes, and Oxidative Stress in Zebrafish (Danio rerio)
| Autor: | Stanislava Stepanova, Misa Skoric, Jana Blahova, Petr Marsalek, Iveta Bedanova, Eva Praskova, Lucie Chromcova, Zdenka Svobodova, Eva Voslarova, Lucie Plhalova, Martin Hostovsky |
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| Rok vydání: | 2014 |
| Předmět: |
Diclofenac
Article Subject Glutathione reductase Danio lcsh:Medicine Pharmacology medicine.disease_cause lcsh:Technology General Biochemistry Genetics and Molecular Biology Lipid peroxidation chemistry.chemical_compound medicine Animals Body Size lcsh:Science Zebrafish General Environmental Science biology lcsh:T lcsh:R General Medicine Glutathione biology.organism_classification Oxidative Stress stomatognathic diseases chemistry Fish growth lcsh:Q sense organs Water Pollutants Chemical Oxidative stress Research Article medicine.drug |
| Zdroj: | The Scientific World Journal, Vol 2014 (2014) The Scientific World Journal |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | The aim of this study was to investigate effects of subchronic exposure to sublethal levels of diclofenac on growth, oxidative stress, and histopathological changes inDanio rerio. The juvenile growth tests were performed onDanio rerioaccording to OECD method number 215. Fish at the age of 20 days were exposed to the diclofenac environmental concentration commonly detected in the Czech rivers (0.02 mg L−1) and the range of sublethal concentrations of diclofenac (5, 15, 30, and 60 mg L−1) for 28 days. A significant decrease (P<0.01) in the fish growth caused by diclofenac was observed in the concentrations of 30 and 60 mg L−1. The identified value of LOEC (lowest observed effect concentration) was 15 mg L−1of diclofenac and NOEC (no observed effect concentration) value was 5 mg L−1of diclofenac. We did not find histopathological changes and changes of selected parameters of oxidative stress (glutathione S-transferase, glutathione reductase) in tested fish. The environmental concentration of diclofenac in Czech rivers did not have any effect on growth, selected oxidative stress parameters (glutathione S-transferase, glutathione reductase), or histopathological changes inDanio reriobut it could have an influence on lipid peroxidation. |
| Databáze: | OpenAIRE |
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