mRNA-Expression of ERα, ERβ, and PR in Clonal Stem Cell Cultures Obtained from Human Endometrial Biopsies
| Autor: | L. Kiesel, J. Braun, Martin Götte, Andreas N. Schüring, S. Wüllner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
medicine.medical_specialty
Stromal cell Article Subject Biopsy and endometriosis lcsh:Medicine Estrogen receptor Biology Endometrium Real-Time Polymerase Chain Reaction lcsh:Technology General Biochemistry Genetics and Molecular Biology progesterone receptor Andrology Internal medicine Progesterone receptor medicine Estrogen Receptor beta Humans RNA Messenger lcsh:Science Estrogen receptor beta Cells Cultured General Environmental Science lcsh:T Stem Cells lcsh:R Estrogen Receptor alpha General Medicine Endocrinology medicine.anatomical_structure lcsh:Q Female Stem cell Receptors Progesterone Estrogen receptor alpha Adult stem cell Research Article estrogen receptor |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 11, Pp 1762-1769 (2011) |
| DOI: | 10.1100/2011/949823 |
| Popis: | Background. Proliferation and differentiation of the endometrium are regulated by estrogen and progesterone. The enormous regenerative capacity of the endometrium is thought to be based on the activity of adult stem cells. However, information on endocrine regulatory mechanisms in human endometrial stem cells is scarce. In the present study, we investigated the expression of ERα, ERβ, and PR in clonal cultures of human endometrial stem cells derived from transcervical biopsies.Methods. Endometrial tissue of 11 patients was obtained by transcervical biopsy. Stromal cell suspensions were plated at clonal density and incubated for 15 days. Expression of ERα, ERβand PR was determined by qPCR prior to and after one cloning round, and normalized to 18 S rRNA expression.Results. Expression of ERαand ERβwas downregulated by 64% and 89%, respectively ( and ). In contrast, PR was not significantly downregulated, due to a more heterogenous expression pattern.Conclusions. Culture of human endometrial stroma cells results in a downregulation of ERαand ERβ, while expression of PR remained unchanged in our patient collective. These results support the hypothesis that stem cells may not be subject to direct stimulation by sex steroids, but rather by paracrine mechanisms within the stem cell niche. |
| Databáze: | OpenAIRE |
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