Broad and Largely Concordant Molecular Changes Characterize Tolerogenic and Immunogenic Dendritic Cell Maturation in Thymus and Periphery

Autor: Thien-Phong Vu Manh, Bjarne Bogen, Bernard Malissen, Karine Crozat, Audrey Jorquera, Elena Tomasello, Hiroaki Azukizawa, Claude Grégoire, Hervé Luche, Laurence Ardouin, Sabrina Carpentier, Rabie Chelbi, Sandrine Henri, Marc Dalod, Samira Tamoutounour, Pierre Grenot, Yannick O. Alexandre, Frédéric Montanana Sanchis, Marc Bajénoff, Anissa Fries, Alaa Shawket, Camille Santa Maria, Even Fossum
Přispěvatelé: Centre d'Immunologie de Marseille - Luminy ( CIML ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Centre d'Immunophénomique ( CIPHE ), Mi-mAbs (C/O CIML), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Unité de recherche Virologie et Immunologie Moléculaires ( VIM ), Institut National de la Recherche Agronomique ( INRA ), Center for Immune Regulation - University of Oslo & Oslo University Hospital Rikshospitalet, University of Oslo ( UiO ), Department of Dermatology [Osaka, Japan], Osaka University [Japan], This work was supported by CNRS, INSERM, PHENOMIN, the European Research Council (FP7/2007–2013 grants 322465 to B.M and 281225 to M.D.), the DCBiol Labex (ANR-11-LA-BEX-0043, grant ANR-10-IDEX-0001-02 PSL), the A*MIDEX project (ANR-11-IDEX-0001-02), the I2HD CIML-SANOFI project, fellowships from the SYBILLA European collaborative project (H.L.) and the I2HD CIML-SANOFI project (A.S.), and grant ANR-11-DPBS-002 (S.C.). This work benefited from data assembled by the ImmGen consortium., ANR-10-IDEX-0001-02/10-IDEX-0001,PSL,PSL ( 2010 ), ANR-11-IDEX-0001-02/11-IDEX-0001,Amidex,Amidex ( 2011 ), ANR-11-DPBS-0002/11-DPBS-0002,MI-mAbs (ex: CIMTECH),MI-mAbs (ex: CIMTECH) ( 2011 ), Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre d'Immunophénomique (CIPHE), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Center for Immune Regulation [Oslo] (CIR), Faculty of Medicine [Oslo], University of Oslo (UiO)-University of Oslo (UiO)-Rigshospitalet [Copenhagen], Copenhagen University Hospital-Copenhagen University Hospital, Osaka University [Osaka], ANR-10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), ANR-11-DPBS-0002,MI-mAbs (ex: CIMTECH),Antibodies for medicine(2011), HENRI, Sandrine, Initiative d'excellence - Paris Sciences et Lettres - - PSL2010 - ANR-10-IDEX-0001 - IDEX - VALID, INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE - - Amidex2011 - ANR-11-IDEX-0001 - IDEX - VALID, Démonstrateurs - Antibodies for medicine - - MI-mAbs (ex: CIMTECH)2011 - ANR-11-DPBS-0002 - DPBS - VALID, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Unité de recherche Virologie et Immunologie Moléculaires (VIM), ANR-10-IDEX-0001-02/10-IDEX-0001,PSL,Paris Sciences et Lettres(2010), ANR-11-IDEX-0001-02/11-IDEX-0001,AMIDEX,AMIDEX(2011), ANR-11-DPBS-0002/11-DPBS-0002,MI-mAbs (ex: CIMTECH),MI-mAbs (ex: CIMTECH)(2011)
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
XCR1
[SDV.IMM] Life Sciences [q-bio]/Immunology
T cell
Immunology
Antigen presentation
chemical and pharmacologic phenomena
Thymus Gland
Biology
Lymphocyte Activation
Virus Replication
T-Lymphocytes
Regulatory
03 medical and health sciences
Mice
0302 clinical medicine
medicine
Immunology and Allergy
Animals
[ SDV.IMM ] Life Sciences [q-bio]/Immunology
Receptor
Gene
Cells
Cultured
Mice
Knockout
Antigen Presentation
Peripheral Tolerance
Toll-Like Receptors
Cell Differentiation
hemic and immune systems
Dendritic cell
Dendritic Cells
Mice
Inbred C57BL
030104 developmental biology
Infectious Diseases
medicine.anatomical_structure
Viral replication
Central Tolerance
Interferon Regulatory Factors
[SDV.IMM]Life Sciences [q-bio]/Immunology
Receptors
Chemokine
Central tolerance
Transcriptome
030215 immunology
Zdroj: Immunity
Immunity, Elsevier, 2016, 45 ((2)), pp.305-18. 〈10.1016/j.immuni.2016.07.019〉
Immunity, Elsevier, 2016, 45 ((2)), pp.305-18. ⟨10.1016/j.immuni.2016.07.019⟩
Immunity, 2016, 45 ((2)), pp.305-18. ⟨10.1016/j.immuni.2016.07.019⟩
ISSN: 1074-7613
Popis: International audience; Dendritic cells (DCs) are instrumental in the initiation of T cell responses, but how thymic and peripheral tolerogenic DCs differ globally from Toll-like receptor (TLR)-induced immunogenic DCs remains unclear. Here, we show that thymic XCR1(+) DCs undergo a high rate of maturation, accompanied by profound gene-expression changes that are essential for central tolerance and also happen in germ-free mice. Those changes largely overlap those occurring during tolerogenic and, more unexpectedly, TLR-induced maturation of peripheral XCR1(+) DCs, arguing against the commonly held view that tolerogenic DCs undergo incomplete maturation. Interferon-stimulated gene (ISG) expression was among the few discriminators of immunogenic and tolerogenic XCR1(+) DCs. Tolerogenic XCR1(+) thymic DCs were, however, unique in expressing ISGs known to restrain virus replication. Therefore, a broad functional convergence characterizes tolerogenic and immunogenic XCR1(+) DC maturation in the thymus and periphery, maximizing antigen presentation and signal delivery to developing and to conventional and regulatory mature T cells.
Databáze: OpenAIRE
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