Doxycycline induces bone repair and changes in Wnt signalling
| Autor: | Paula Goes Pinheiro Dutra, Ana Paula Negreiros Nunes Alves, Glauce Socorro de Barros Viana, Kátia do Nascimento Gomes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
alveolar bone loss Male Osteoclasts Inflammation Bone healing Matrix metalloproteinase Andrology Immunoenzyme Techniques Osso e Ossos 03 medical and health sciences Dickkopf-1 0302 clinical medicine medicine Alveolar Process Animals Rats Wistar General Dentistry Wnt Signaling Pathway Dental alveolus Doxycycline Wound Healing Osteoblasts doxycycline business.industry 030206 dentistry bone repair Molar Staining Rats Doxiciclina CTL Disease Models Animal 030104 developmental biology bone diseases Immunology immunohistochemistry Tooth Extraction Wnt-10b Original Article Bone Remodeling medicine.symptom business Immunostaining medicine.drug |
| Zdroj: | International Journal of Oral Science Repositório Institucional da Universidade Federal do Ceará (UFC) Universidade Federal do Ceará (UFC) instacron:UFC |
| ISSN: | 2049-3169 1674-2818 |
| Popis: | Doxycycline (DOX) exhibits anti-in fl ammatory and MMP inhibitory properties. The objectives of this study were to evaluate the effects of DOX on alveolar bone repair. Controls (CTL) and DOX-treated (10 and 25 mg . kg − 1 ) molars were extracted, and rats were killed 7 or 14 days later. The maxillae were processed and subjected to histological and immunohistochemical assays. Hematoxylin-eosin staining (7th day) revealed in fl ammation in the CTL group that was partly reversed after DOX treatment. On the 14th day, the CTL group exhibited bone neoformation, conjunctive tissue, re-epithelization and the absence of in fl ammatory in fi ltrate. DOX-treated groups exhibited complete re-epithelization, tissue remodelling and almost no in fl ammation. Picrosirius red staining in the DOX10 group (7th and 14th days) revealed an increased percentage of type I and III collagen fi bres compared with the CTL and DOX25 groups. The DOX10 and DOX25 groups exhibited increases in osteoblasts on the 7th and 14th days. However, there were fewer osteoclasts in the DOX10 and DOX25 groups on the 7th and 14th days. Wnt-10b- immunopositive cells increased by 130% and 150% on the 7th and 14th days, respectively, in DOX-treated groups compared with the CTL group. On the 7th day, Dickkopf (Dkk)-1 immunostaining was decreased by 63% and 46% in the DOX10 and DOX25 groups, respectively. On the 14th day, 69% and 42% decreases in immunopositive cells were observed in the DOX10 and DOX25 groups, respectively, compared with the CTL group. By increasing osteoblasts, decreasing osteoclasts, activating Wnt 10b and neutralising Dkk, DOX is a potential candidate for bone repair in periodontal diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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