Separation of seventeen 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans and 12 dioxin-like polychlorinated biphenyls by comprehensive two-dimensional gas chromatography with electron-capture detection
| Autor: | Conny Danielsson, P.E.G. Leonards, Peter Korytár, U.A.Th. Brinkman, J. de Boer, Peter Haglund |
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| Rok vydání: | 2004 |
| Předmět: |
Chromatography
Gas Polychlorinated Dibenzodioxins Electron capture pcbs Sensitivity and Specificity Biochemistry Analytical Chemistry contamination spain samples Benzofurans Chromatography Chemistry Organic Chemistry General Medicine Dibenzofurans Polychlorinated Contamination RIVO Milieu en Voedselveiligheid congeners Electron capture detector Two-dimensional chromatography catalonia Polychlorinated Dibenzo-p-dioxins Environmental chemistry Two-dimensional gas Gas chromatography Polychlorinated dibenzofurans |
| Zdroj: | Journal of Chromatography. A, Including electrophoresis and other separation methods, 1038, 189-199 Journal of Chromatography. A, Including electrophoresis and other separation methods 1038 (2004) |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2004.03.026 |
| Popis: | Comprehensive two-dimensional gas chromatography (GC x GC) with electron-capture detection (ECD) has been optimized for the separation of seventeen 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans and 12 dioxin-like polychlorinated biphenyls, with emphasis on the selection of the first- and second-dimension, commercially available, columns. When eight second-dimension columns were subsequently combined with a 100% methylpolysiloxane stationary phase (DB-1) in the first dimension to create orthogonal conditions, a complete separation of all congeners with different TEF values was obtained with two column combinations, DB-1 x VF-23 and DB-1 x LC-50. When other types of first-dimension columns were used (and orthogonality was partly sacrificed), a DB-XLB column combined with 007-65HT, VF-23 and LC-50 was found to provide a complete separation of all 29 priority congeners. Next, the potential of these three column combinations for real-life analysis was preliminarily studied. With a spiked and fractionated milk extract, DB-XLB x LC-50 was found to be the most powerful column combination, because of the good separation of the 29 priority congeners from each other as well as from the matrix constituents. Quantitative performance (close to three-order linearity; LODs, 30-150 fg injected; R.S.D.s, 1.5-6.5% (n = 10)) was satisfactory. |
| Databáze: | OpenAIRE |
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