Platelets modulate the immune response following trauma by interaction with CD4+ T regulatory cells in a mouse model

Autor: Friederike Hefele, Stefan Huber-Wagner, Christian B. Bergmann, Marina Unger, Marc Hanschen, Martijn van Griensven, Peter Biberthaler
Rok vydání: 2015
Předmět:
0301 basic medicine
CD4(+) T-CELLS
Male
BLOOD
P-selectin
T-Lymphocytes
Cell Communication
Lymphocyte Activation
T-Lymphocytes
Regulatory
Mice
T-Lymphocyte Subsets
Homeostasis
Platelet
Hemostatic function
NEUTROPHIL EXTRACELLULAR TRAPS
hemic and immune systems
Regulatory T cells
PKC-THETA
Acquired immune system
T-Lymphocyte Subsets/immunology
P-Selectin
P-Selectin/metabolism
medicine.symptom
DEPLETION
Adaptive immune response
Burns
CRITICALLY-ILL PATIENTS
Platelets
Blood Platelets
Immunology
chemical and pharmacologic phenomena
Inflammation
T-Lymphocytes
Regulatory/immunology
Lymphocyte Activation/immunology
Trauma
Immunomodulation
03 medical and health sciences
Toll-Like Receptor 9/metabolism
Immune system
INFLAMMATION
medicine
INJURY
Animals
Platelet activation
Blood Platelets/immunology
Hemostasis
SEPSIS
business.industry
Animal
Tetraspanin 30
Burns/immunology
PSEUDOMONAS-AERUGINOSA
Neutrophil extracellular traps
Disease Models
Animal
030104 developmental biology
Toll-Like Receptor 9
Disease Models
Tetraspanin 30/metabolism
Wounds and Injuries
Regulatory/immunology
Wounds and Injuries/immunology
business
Biomarkers
Zdroj: Immunologic Research, 64(2), 508-517. Humana Press, Inc.
ISSN: 1559-0755
0257-277X
Popis: CD4+ T regulatory cells (Tregs) play a pivotal role in the anti-inflammatory immune response following trauma. The mechanisms of CD4+ Treg activation are mostly unknown. Here, we hypothesize that platelets regulate CD4+ Treg activation following trauma. In a murine burn injury model (male C57Bl/6N mice), depletion of platelets or CD4+ Tregs was conducted. Draining lymph nodes, blood and spleen were harvested 2 h and 7 days after trauma. CD4+ Treg activation was measured using phospho- and conventional flow cytometry. Platelet activation was analyzed using thromboelastometry and flow cytometry. Trauma differentially activates CD4+ T cells, early after trauma only CD4+ Tregs are activated. Following burn injury, platelets augment the activation of CD4+ Tregs. This effect could only be seen early after trauma. While CD4+ Tregs influence hemostasis early following trauma, platelet activation markers were unchanged. Beyond their role in hemostasis, platelets are able to modulate the immunologic host response to trauma-induced injury by augmenting the activation of CD4+ Tregs. CD4+ Treg activation following trauma is considered protective. In addition, CD4+ Tregs are capable of modulating the hemostatic function of platelets. For the first time, we could show reciprocal activation of platelets and CD4+ Tregs as part of the protective immune response following trauma.
Databáze: OpenAIRE
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