Inhibitory action of palatinose and its hydrogenated derivatives on the hydrolysis of alpha-glucosylsaccharides in the small intestine
| Autor: | Jun Kashimura, Yukie Nagai, Toshinao Goda |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Sucrose
Disaccharide Sucrase-isomaltase complex Sucrase chemistry.chemical_compound Hydrolysis Intestine Small Carbohydrate Conformation Glycoside Hydrolase Inhibitors Enzyme Inhibitors Maltose chemistry.chemical_classification alpha-Glucosidases General Chemistry Isomaltose Sucrase-Isomaltase Complex carbohydrates (lipids) chemistry Biochemistry Dextrin Hydrogenation General Agricultural and Biological Sciences |
| Zdroj: | Journal of agricultural and food chemistry. 56(14) |
| ISSN: | 1520-5118 |
| Popis: | This study was conducted to investigate the inhibitory effects of palatinose and Palatinit, which are disaccharides (or disaccharide alcohol) connected through an alpha-1,6-glucosyl linkage, on the hydrolysis of other carbohydrates, using an enzyme extract from the rat small intestine and a purified sucrase-isomaltase complex. Palatinose and its hydrogenated product, Palatinit, an equimolar mixture of alpha-O-D-glucopyranosyl-1,6-D-sorbitol (GPS) and alpha-O-D-glucopyranosyl-1,6-D-mannitol (GPM), inhibited the hydrolysis of sucrose and maltose. Palatinose and Palatinit also inhibited the hydrolysis of dextrin and soluble starch. Kinetic analysis of the enzymatic inhibition by GPS and GPM on sucrose hydrolysis revealed that both GPS and GPM competitively inhibit sucrase catalytic activity. These results suggest that disaccharides with an alpha-1,6-glucosyl linkage competitively inhibit intestinal alpha-glucosidases and may reduce the rate of hydrolysis of sucrose and other alpha-glucosylsaccharides. |
| Databáze: | OpenAIRE |
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