Programmed cortical ER collapse drives selective ER degradation and inheritance in yeast meiosis
| Autor: | Gloria A. Brar, Jessica Mae Leslie, Tia Cheunkarndee, George Maxwell Otto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Inheritance Patterns Saccharomyces cerevisiae Biology Endoplasmic Reticulum Time-Lapse Imaging Article Imaging Three-Dimensional Meiosis Macroautophagy medicine Autophagy Compartment (development) Transcription factor Anaphase Organelles Endoplasmic reticulum Cell Membrane Cell Biology Actin cytoskeleton Endoplasmic Reticulum Stress Autophagic Punctum Cell biology Actin Cytoskeleton medicine.anatomical_structure Germ Cells Mutation Gamete Carrier Proteins Cell Cycle and Division |
| Zdroj: | The Journal of Cell Biology Autophagy |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Otto et al. show that the cortical ER undergoes programmed collapse from the plasma membrane during meiosis. Collapse separates the ER into distinct populations and determines which regions are eligible for inheritance by gametes and which are degraded by mechanisms including Atg40-mediated autophagy. The endoplasmic reticulum (ER) carries out essential and conserved cellular functions, which depend on the maintenance of its structure and subcellular distribution. Here, we report developmentally regulated changes in ER morphology and composition during budding yeast meiosis, a conserved differentiation program that gives rise to gametes. A subset of the cortical ER collapses away from the plasma membrane at anaphase II, thus separating into a spatially distinct compartment. This programmed collapse depends on the transcription factor Ndt80, conserved ER membrane structuring proteins Lnp1 and reticulons, and the actin cytoskeleton. A subset of ER is retained at the mother cell plasma membrane and excluded from gamete cells via the action of ER–plasma membrane tethering proteins. ER remodeling is coupled to ER degradation by selective autophagy, which relies on ER collapse and is regulated by timed expression of the autophagy receptor Atg40. Thus, developmentally programmed changes in ER morphology determine the selective degradation or inheritance of ER subdomains by gametes. Graphical Abstract |
| Databáze: | OpenAIRE |
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