Understanding substrate binding and the role of gatekeeping residues in PigC access tunnels
| Autor: | Stefanie Brands, Anna Joëlle Ruff, Ulrich Schwaneberg, Hannah U. C. Brass, Jarno G. Sikkens, Andreas Sebastian Klein, Mehdi D. Davari, Jörg Pietruszka |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
chemistry.chemical_classification
0303 health sciences DNA ligase 010405 organic chemistry Chemistry Stereochemistry Metals and Alloys Binding pocket Substrate (chemistry) General Chemistry 01 natural sciences Catalysis 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials 03 medical and health sciences ddc:540 Materials Chemistry Ceramics and Composites Catalytic efficiency 030304 developmental biology |
| Zdroj: | Chemical communications 57(21), 2681-2684 (2021). doi:10.1039/D0CC08226K |
| Popis: | Semi-rational redesign of the substrate binding pocket and access tunnels of prodigiosin ligase PigC enhanced the catalytic efficiency in the synthesis of pyrrolic anti-cancer agents more than 45 times. A molecular understanding was gained on residues V333 and T334 relevant to substrate binding and translocation of small pyrroles through PigC access tunnels. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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