| Autor: |
Konč, Juraj, Brown, Libby, Whiten, Daniel R, Zuo, Yukun, Ravn, Peter, Klenerman, David, Bernardes, Gonçalo JL |
| Přispěvatelé: |
Ravn, Peter [0000-0003-3032-9037], Bernardes, Gonçalo JL [0000-0001-6594-8917], Apollo - University of Cambridge Repository |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.17863/cam.76109 |
| Popis: |
Many bioconjugation strategies for DNA oligonucleotides and antibodies suffer limitations, such as site-specificity, stoichiometry and hydrolytic instability of the conjugates, which makes them unsuitable for biological applications. Here, we report a new platform for the preparation of DNA-antibody bioconjugates with a simple benzoylacrylic acid pentafluorophenyl ester reagent. Benzoylacrylic-labelled oligonucleotides prepared with this reagent can be site-specifically conjugated to a range of proteins and antibodies through accessible cysteine residues. The homogeneity of the prepared DNA-antibody bioconjugates was confirmed by a new LC-MS protocol and the bioconjugate probes were used in fluorescence or super-resolution microscopy cell imaging experiments. This work demonstrates the versatility and robustness of our bioconjugation protocol that gives site-specific, well-defined and plasma-stable DNA-antibody bioconjugates for biological applications. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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