Distinctions among Circulating Antibody-Secreting Cell Populations, Including B-1 Cells, in Human Adult Peripheral Blood
| Autor: | Thomas L. Rothstein, Nely Rodríguez-Zhurbenko, Xiaoti Guo, Thomas J. Hopkins, Tam D. Quach, Wentian Li, Ana María Hernández |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male 0301 basic medicine medicine.medical_treatment Immunology Population Naive B cell B-Lymphocyte Subsets Cell Separation CD38 Article Immunophenotyping Young Adult 03 medical and health sciences Antigen Antigens CD immune system diseases hemic and lymphatic diseases medicine Humans Immunology and Allergy education B cell Aged Oligonucleotide Array Sequence Analysis Aged 80 and over CD20 education.field_of_study biology Reverse Transcriptase Polymerase Chain Reaction hemic and immune systems Middle Aged Flow Cytometry Molecular biology 030104 developmental biology medicine.anatomical_structure Cytokine biology.protein Female Immunologic Memory |
| Zdroj: | The Journal of Immunology. 196:1060-1069 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Human antibody secreting cell (ASC) populations in circulation are not well studied. In addition to B-1 (CD20+CD27+CD38lo/intCD43+) cell and the conventional plasmablast (CD20-CD27hiCD38hi) cell populations, here we identified a novel B cell population termed 20+38hi B cells (CD20+CD27hiCD38hi) that spontaneously secretes antibody. At steady state, 20+38hi B cells are distinct from plasmablasts on the basis of CD20 expression, amount of antibody production, frequency of mutation, and diversity of B cell receptor repertoire. However, cytokine treatment of 20+38hi B cells induces loss of CD20 and acquisition of CD138, suggesting that 20+38hi B cells are precursors to plasmablasts, or pre-plasmablasts. We then evaluated similarities and differences between CD20+CD27+CD38lo/intCD43+ B-1 cells, CD20+CD27hiCD38hi 20+38hi B cells, CD20-CD27hiCD38hi plasmablasts, and CD20+CD27+CD38lo/intCD43- memory B cells. We found that B-1 cells differ from 20+38hi B cells and plasmablasts in numbers of ways, including antigen expression, morphological appearance, transcriptional profiling, antibody skewing, antibody repertoire, and secretory response to stimulation. In terms of gene expression, B-1 cells align more closely with memory B cells than with 20+38hi B cells or plasmablasts, but differ in that memory B cells do not express antibody secretion related genes. We found that, B-1 cell antibodies utilize Vh4-34, which is often associated with autoreactivity, 3 to 6-fold more often than other B cell populations. Along with selective production of IgM anti-PC, this data suggests that human B-1 cells might be preferentially selected for autoreactivity/natural-specificity. In sum, our results indicate that human healthy adult peripheral blood at steady state consists of 3 distinct ASC populations. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|