In vitro histomechanical effects of enzymatic degradation in carotid arteries during inflation tests with pulsatile loading

Autor: Norbert Laroche, Stéphane Avril, O. Trabelsi, Edouard Breysse, Virginie Dumas
Přispěvatelé: Biomécanique et Bioingénierie (BMBI), Université de Technologie de Compiègne (UTC)-Centre National de la Recherche Scientifique (CNRS), INSERM U1059, SAINBIOSE - Santé, Ingénierie, Biologie, Saint-Etienne (SAINBIOSE-ENSMSE), Centre Ingénierie et Santé (CIS-ENSMSE), École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-École des Mines de Saint-Étienne (Mines Saint-Étienne MSE), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université Jean Monnet [Saint-Étienne] (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Tribologie et Dynamique des Systèmes (LTDS), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École Nationale des Travaux Publics de l'État (ENTPE)-Ecole Nationale d'Ingénieurs de Saint Etienne-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Swine
Carotid arteries
Sus scrofa
Biomedical Engineering
Pulsatile flow
FOS: Physical sciences
02 engineering and technology
Biomaterials
03 medical and health sciences
0302 clinical medicine
In vivo
collagen proteolysis
medicine
Animals
Collagenases
Tissues and Organs (q-bio.TO)
histomechanical effects
Chemistry
Stiffness
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
Quantitative Biology - Tissues and Organs
030206 dentistry
021001 nanoscience & nanotechnology
Physics - Medical Physics
In vitro
Biomechanical Phenomena
Carotid Arteries
Mechanics of Materials
FOS: Biological sciences
Collagenase
Axial load
Medical Physics (physics.med-ph)
Collagen
Stress
Mechanical
pulsatile/inflation tests
medicine.symptom
0210 nano-technology
damage
Carotid artery
medicine.drug
Biomedical engineering
Enzymatic degradation
Zdroj: Journal of the mechanical behavior of biomedical materials
Journal of the mechanical behavior of biomedical materials, Elsevier, 2020, 103, pp.103550. ⟨10.1016/j.jmbbm.2019.103550⟩
Journal of the Mechanical Behavior of Biomedical Materials
ISSN: 1751-6161
1878-0180
DOI: 10.1016/j.jmbbm.2019.103550⟩
Popis: International audience; In this paper, the objective is to assess the histomechanical effects of collagen proteolysis in arteries under loading conditions reproducing in vivo environment. Thirteen segments of common porcine carotid arteries (8 proximal and 5 distal) were immersed in a bath of bacterial collagenase and tested with a pulsatile tension/inflation machine. Diameter, pressure and axial load were monitored throughout the tests and used to derive the stress-stretch curves and to determine the secant circumferential stiffness. Results were analyzed separately for proximal and distal segments, before and after 1, 2 and 3 hours of enzymatic degradation. A histological analysis was performed to relate the arterial microstructure to its mechanical behavior under collagen proteolysis. Control (before enzymatic degradation) and treated populations (after 1, 2 or 3 hours of enzymatic degradation) were found statistically incomparable, and histology confirmed the alteration of the fibrous structure of collagen bundles induced by the collagenase treatment. A decrease of the secant circumferential stiffness of the arterial wall was noticed mostly at the beginning of the treatment, and was less pronounced after 1 hour. These results constitute an important set of enzymatically damaged arteries that can be used to validate biomechanical computational models correlating structure and properties of blood vessels.
Databáze: OpenAIRE
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