Role of interaction of CD2 molecules with lymphocyte function-associated antigen 3 in T-cell recognition of nominal antigen
| Autor: | Trebor Lawton, Barbara P. Wallner, Michael A. Recny, David Novick, Robert F. Siliciano, Ellis L. Reinherz, Shigeo Koyasu |
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| Rok vydání: | 1990 |
| Předmět: |
Antigens
Differentiation T-Lymphocyte T-Lymphocytes Antigen presentation CD2 Antigens Receptors Antigen T-Cell chemical and pharmacologic phenomena Cell Communication Biology Lymphocyte Activation Major histocompatibility complex Mice L Cells Antigen Antigens CD Animals Humans Cytotoxic T cell Receptors Immunologic Cells Cultured Membrane Glycoproteins Multidisciplinary Antigen processing T-cell receptor Antibodies Monoclonal T lymphocyte MHC restriction CD58 Antigens Molecular biology Clone Cells Intercellular Junctions Antigens Surface biology.protein Peptides Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences. 87:2603-2607 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | The role of the interaction of CD2 molecules with lymphocyte function-associated antigen 3 (LFA-3) in facilitating nominal antigen recognition by T lymphocytes was studied by utilizing an HLA-DR4-restricted CD4+ cytotoxic human T-cell clone specific for human immunodeficiency virus envelope glycoprotein gp120 as a responder and murine fibroblasts transfected with human class II major histocompatibility complex (MHC) and/or human LFA-3 molecules as antigen-presenting cells (APC). Although expression of the DR4 restriction element in fibroblasts is sufficient for T-cell recognition of a gp120 peptide as judged by induction of proliferation coexpression of human LFA-3 on DR4+ APC decreases the molar requirement of nominal antigen by greater than one order of magnitude. Both LFA-3 and the relevant class II MHC molecules are necessary for antigen-independent conjugate formation, but the binding is further enhanced by specific nominal antigen. CD2-LFA-3 interaction is independent of T-cell receptor-MHC interaction and contributes directly to the stabilized conjugate between the T cell and LFA-3-bearing APC; soluble CD2 and monoclonal antibodies to LFA-3 and CD2 reduce T-cell-APC binding to the level mediated by nominal antigen and MHC. During conjugate formation, CD2 but not CD3 molecules are reorganized into the cell-cell interaction site in an antigen-independent manner. Thus, reorganization and/or coassociation of CD2 with CD3 molecules is not essential for T-cell activation. |
| Databáze: | OpenAIRE |
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