| Autor: |
Yi Xu, Peihuan He, Waqas Ahmad, Md Mehedi Hassan, Shujat Ali, Huanhuan Li, Quansheng Chen |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biosensors and Bioelectronics. 209:114240 |
| ISSN: |
0956-5663 |
| DOI: |
10.1016/j.bios.2022.114240 |
| Popis: |
Staphylococcus aureus (S. aureus) has been identified as a marker of food contamination, closely associated with human health. This work designs a sensitive and rapid bio-detection strategy for S. aureus based on hybridization chain reaction-assisted surface enhanced Raman scattering (HCR-assisted-SERS) signal amplification. In this approach, the interaction between the aptamer (Apt) and its partial complementary DNA strands (cDNA) fabricated on the surface of gold-assisted magnetic nanoparticles (Au-MNPs) and the subsequent detachment of the cDNA results in the activation of the HCR process. In the HCR, a pair of hairpin structured DNA probes (H1 and H2) with sticky ends self-assembles to form a long DNA polymer. Subsequently, the output and amplification of the SERS signal were performed by conjugating 4-ATP modified Au@Ag NPs with the obtained DNA polymer via a specific Ag-S bond, and further collected through a self-administered polydimethylsiloxane (PDMS) cone-shaped support array. The precise quantification of S. aureus was performed in the concentration range of 28 to 2.8 × 10 |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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