The attenuating effects of plumbagin on pro-inflammatory cytokine expression in LPS-activated BV-2 microglial cells
Autor: | Malak G. Kolta, Karam F.A. Soliman, Samia S. Messeha, Najla O. Zarmouh, Patricia Mendonca |
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Rok vydání: | 2017 |
Předmět: |
Lipopolysaccharides
0301 basic medicine medicine.drug_class Immunology Anti-Inflammatory Agents Nitric Oxide Synthase Type II Biology Pharmacology Nitric Oxide Neuroprotection Article Anti-inflammatory Nitric oxide Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Extracellular Animals Immunology and Allergy Cytokine Antibody IC50 Cell Line Transformed Dose-Response Relationship Drug Microglia Lysine Plumbagin 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Neurology chemistry Cytokines Neurology (clinical) 030217 neurology & neurosurgery Naphthoquinones |
Zdroj: | Journal of Neuroimmunology. 313:129-137 |
ISSN: | 0165-5728 |
Popis: | Activated microglial cells produce the pro-inflammatory mediators such as nitric oxide (NO) and cytokines. The excessive release of these mediators can lead to neurodegenerative diseases, such as Alzheimer's disease (AD) and Parkinson's disease (PD). Inhibition of the release of these pro-inflammatory molecules may prevent or halt the progression of these diseases. Plumbagin (PL), a naphthoquinone compound in the roots of the traditional medicinal plant Plumbago zeylanica L., showed anti-inflammatory effects on macrophages. However, PL effects on activated microglia remain unknown. In the present study, PL has been examined for its anti-inflammatory effect on LPS – activated microglial BV-2 cells. In this study, NO and iNOS expression were investigated in BV-2 microglial cells in the presence of PL or the selective iNOS inhibitor L-N6-(1-iminoethyl) lysine (L-NIL). The results obtained indicate that PL was > 30-fold potent than L-NIL in inhibiting NO production with an IC50 of 0.39 μM. Our immunofluorescence study confirmed the ability of PL to significantly inhibit iNOS expression in the activated microglia. Furthermore, the extracellular microglial pro-inflammatory cytokine expression in the presence of 2 μM of PL was detected, quantified, and validated using cytokine antibody protein arrays and quantitative ELISA. The results obtained showed that PL significantly downregulated the expression of many cytokines including IL-1α, G-CSF, IL-12 p40/p70, MCP-5, MCP-1, and IL-6. In conclusion, PL potency in attenuating multiple pro-inflammatory agents indicates its potential to be used for neurodegenerative diseases. |
Databáze: | OpenAIRE |
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