Proteomes of the female salivary glands of Simulium nigrogilvum and Simulium nodosum, the main human-biting black flies in Thailand
| Autor: | Kritsana Taai, Chayanit Hempolchom, Narissara Jariyapan, Onrapak Reamtong, Nitat Sookrung, Watcharatip Dedkhad, Atiporn Saeung, Yuwaporn Sakolvaree, Hiroyuki Takaoka, Wanpen Chaicumpa, Wichai Srisuka |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Proteases Nematocera Proteome Veterinary (miscellaneous) 030231 tropical medicine Aminopeptidase Salivary Glands Microbiology 03 medical and health sciences 0302 clinical medicine Species Specificity Animals Humans Simuliidae Salivary Proteins and Peptides Simulium Polyacrylamide gel electrophoresis chemistry.chemical_classification biology Insect Bites and Stings 030108 mycology & parasitology Thailand biology.organism_classification Infectious Diseases Enzyme Gene Expression Regulation chemistry Insect Science Electrophoresis Polyacrylamide Gel Female Parasitology Black fly Chromatography Liquid |
| Zdroj: | Acta Tropica. 194:82-88 |
| ISSN: | 0001-706X |
| Popis: | Although several studies have reported pharmacological and immunological activity, as well as the role of black flies in transmitting pathogens to vertebrate hosts through salivary glands (SG) during blood feeding, SG proteomes of the anthropophilic black flies in Thailand have never been reported. Therefore, this study determined the SG proteomes of female S. nigrogilvum and S. nodosum. Sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional (2-DE) gels containing separated SG proteins of individual species were subjected to liquid chromatography-tandem mass spectrometry (LCMS/MS) and an orthologous protein search from eukaryotic organism, nematocera and simuliidae databases for total protein identification. SDS-PAGE and protein staining revealed at least 13 and 9 major protein bands in the SGs of female S. nigrogilvum and S. nodosum, respectively, as well as several minor ones. The 2-DE demonstrated a total of 56 and 41 protein spots for S. nigrogilvum and S. nodosum, respectively. Most of the proteins obtained in both species were enzymes involved in blood feeding, including proteases, apyrases, hyaluronidases, aminopeptidase and elastase. The results obtained in this study provided a new body of knowledge for a better understanding on the role of salivary gland proteins in these black fly species in Thailand. |
| Databáze: | OpenAIRE |
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