Immunohistochemical staining and computed tomography in early detection of lung cancer among heavy smokers: A pilot study
| Autor: | Mohammad Khairy El-Badrawy, Khalied R. Zalata, Abdel-Hady El-Gilany, Ahmad S. El-Morsy, Nesrien M. Shalabi, Amina Soltan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Sputum Cytology
Pathology medicine.medical_specialty Radiography protein p53 H&E stain 03 medical and health sciences 0302 clinical medicine lung cancer screening medicine Lung cancer monoclonal antibody p53 lcsh:RC705-779 Solitary pulmonary nodule medicine.diagnostic_test sputum cytology business.industry lcsh:Medical emergencies. Critical care. Intensive care. First aid chest computed tomography lcsh:Diseases of the respiratory system lcsh:RC86-88.9 respiratory system medicine.disease respiratory tract diseases lung cancer Bronchoalveolar lavage 030228 respiratory system 030220 oncology & carcinogenesis Sputum medicine.symptom business Lung cancer screening |
| Zdroj: | The Egyptian Journal of Bronchology, Vol 11, Iss 2, Pp 149-153 (2017) |
| ISSN: | 2314-8551 1687-8426 |
| DOI: | 10.4103/1687-8426.203800 |
| Popis: | Objectives Early diagnosis of lung cancer carries a good prognosis. The aim of the present study was early detection of lung cancer among heavy smokers using immunohistochemical staining and chest computed tomography (CT). Patients and methods This descriptive cross-sectional study comprised 80 heavy smokers with smoking index more than 40 pack-years. They were recruited from the Smoking Cessation Clinic, Mansoura University Hospital. All participants were subjected to (a) chest radiography followed by high-resolution chest CT, (b) sputum sample collection, and (c) fiberoptic bronchoscopy evaluation of bronchoalveolar lavage (BAL) and bronchial mucosal biopsies from suspicious areas. All pathological samples were stained with hematoxylin and eosin followed by immunostaining using antibodies for p53 and thyroid transcription factor-1 (TTF-1). Data were analyzed using statistical package for social sciences, version 16. Results The majority of heavy smokers were male, withamean age of 53.42±11.30 years. A solitary pulmonary nodule was detectedin1.3%of cases withchest radiographyandin5%with high-resolution CT. Hematoxylin and eosin staining of sputum, BAL, and mucosal biopsies was positive for premalignant changes in 35, 27, and 17.56%of cases, respectively. Sputum, BAL, and mucosal biopsies showed expression of p53 in 30, 37.8, and 35.1% of cases, respectively. Sputum, BAL, and mucosal biopsy showed expression of TTF-1 in 12.5, 10.8, and 14.9% of cases, respectively. Conclusion The immunohistochemical technique using p53 and TTF-1 is useful in the early detection of bronchial mucosal changes in heavy smokers. There is still need for a largescale study to highlight its validity and acceptability. Meanwhile, chest CT is beneficial for the detection of peripheral small lesions. |
| Databáze: | OpenAIRE |
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