Transport of Anti-Glycoprotein IIb/IIIa-Antibodies into the Alpha-Granules of Unstimulated Human Blood Platelets
| Autor: | Heinrich Patscheke, Eberhard Morgenstern, Andreas Ruf |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Blood Platelets
chemistry.chemical_classification medicine.drug_class Chemistry Antibodies Monoclonal Biological Transport Active Prostacyclin Platelet Membrane Glycoproteins Hematology In Vitro Techniques Cytoplasmic Granules Endocytosis Monoclonal antibody Molecular biology Biochemistry medicine Humans Platelet Platelet activation Microscopy Immunoelectron Glycoprotein IIb/IIIa Glycoprotein medicine.drug Iloprost |
| Zdroj: | Thrombosis and Haemostasis. 67:121-125 |
| ISSN: | 2567-689X 0340-6245 |
| Popis: | SummaryThe redistribution of the antibody-glycoprotein (GP) IIb/IIIa complex was investigated with the immuno-gold labeling technique in order to trace its transport in resting platelets. Washed platelets were incubated in the presence of aspirin and a prostacyclin analogue (iloprost) with three different monoclonal antibodies (Gi3, J15 and P2) against GPIIb/IIIa. The examination of ultrathin serial sections showed that the surface labeling was internalized into the surface connected membrane system (SCS). Labels were found within the alpha-granules after 40 min and the number of labels increased during longer incubation periods (max. 120 min). The transport possibly involved coated membranes. The alpha-granules were neither found to be altered during this process nor were any morphological signs of platelet activation detectable. The anti-GPIIb/IIIa complex remained membrane-associated during the transfer. These observations indicate that the membrane-GPIIb/IIIa complex was stable and transported from the surface into the alpha-granules of resting platelets. Since this transport was not influenced by iloprost or by aspirin it may be interpreted as constitutive endocytosis. |
| Databáze: | OpenAIRE |
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