A star-PEG–heparin hydrogel platform to aid cell replacement therapies for neurodegenerative diseases
| Autor: | Andreas Hermann, Andrea Zieris, Katja Stirl, Milauscha Grimmer, Alexander Storch, Woranan Panyanuwat, Sigrid C. Schwarz, Uwe Freudenberg, Dorit Meinhold, Stefan Zschoche, Petra B. Welzel, Carsten Werner |
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| Rok vydání: | 2009 |
| Předmět: |
Materials science
Cell Survival Cell Biophysics Bioengineering Cell fate determination Hydrogel Polyethylene Glycol Dimethacrylate Polyethylene Glycols Biomaterials Mice Tissue engineering Mesencephalon Pregnancy Elastic Modulus PEG ratio medicine Animals Rats Wistar Cell adhesion Cells Cultured Embryonic Stem Cells Neurons Heparin Neurodegenerative Diseases Prostheses and Implants Neural stem cell Rats Mice Inbred C57BL medicine.anatomical_structure Mechanics of Materials Self-healing hydrogels Ceramics and Composites Female Fibroblast Growth Factor 2 Oligopeptides Biomedical engineering medicine.drug |
| Zdroj: | Biomaterials. 30:5049-5060 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2009.06.002 |
| Popis: | Biofunctional matrices for in vivo tissue engineering strategies must be modifiable in both biomolecular composition and mechanical characteristics. To address this challenge, we present a modular system of biohybrid hydrogels based on covalently cross-linked heparin and star-shaped poly(ethylene glycols) (star-PEG) in which network characteristics can be gradually varied while heparin contents remain constant. Mesh size, swelling and elastic moduli were shown to correlate well with the degree of gel component cross-linking. Additionally, secondary conversion of heparin within the biohybrid gels allowed the covalent attachment of cell adhesion mediating RGD peptides and the non-covalent binding of soluble mitogens such as FGF-2. We applied the biohybrid gels to demonstrate the impact of mechanical and biomolecular cues on primary nerve cells and neural stem cells. The results demonstrate the cell type-specific interplay of synergistic signaling events and the potential of biohybrid materials to selectively stimulate cell fate decisions. These findings suggest important future uses for this material in cell replacement based-therapies for neurodegenerative diseases. |
| Databáze: | OpenAIRE |
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