Comparison of the (CCG)4-based PCR and MIRU-VNTR for molecular typing of Mycobacterium avium strains
| Autor: | Ewa Augustynowicz-Kopeć, Arkadiusz Wojtasik, Pawel Parniewski, Anna Krzyzanowska, Anna B. Kubiak, Marta Majchrzak |
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| Rok vydání: | 2011 |
| Předmět: |
Genetics
Genotype Minisatellite Repeat General Medicine Minisatellite Repeats Biology bacterial infections and mycoses biology.organism_classification Mycobacterium avium Complex Polymerase Chain Reaction Molecular Typing Molecular typing Intergenic region Tandem repeat Humans DNA Intergenic Typing Molecular Biology Genotyping Mycobacterium Mycobacterium avium-intracellulare Infection |
| Zdroj: | Molecular biology reports. 39(7) |
| ISSN: | 1573-4978 |
| Popis: | Mycobacterium avium, a member of the group of non-tuberculous mycobacteria, is most often responsible for the serious diseases in humans and is frequently isolated from NTM-caused pulmonary events. In this connection the epidemiological aspect is also of great importance. Here we present a useful genetic assay that uses (CCG)(4)-based PCR for genotyping M. avium. After applying this test to 33 strains of M. avium, we found a discriminatory index of 0.979. The accuracy of this analysis was supported by a reasonable reproducibility of 95.1%. These results were compared with the Mycobacterial Intergenic Repeat Unit-Variable Number Tandem Repeats (MIRU-VNTR) typing scheme which had slightly lower discriminatory index of 0.945 however, the method was able to cluster different strains compared to CCG-PCR. Taking into account high discriminatory index and reproducibility, this test scheme has the potential as a screening tool in the investigation of M. avium infections, especially if combined with MIRU-VNTR. |
| Databáze: | OpenAIRE |
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