Parathyroid hormone receptor stimulation induces human adipocyte lipolysis and browning

Autor: Niels Jessen, Bjørn Richelsen, Steen B. Pedersen, Peter Breining, Jacob B. Hansen, Mads Kjolby
Rok vydání: 2021
Předmět:
Adult
Male
endocrine system
medicine.medical_specialty
Adipose Tissue
Brown/metabolism
Lipolysis
Endocrinology
Diabetes and Metabolism
Receptor expression
Parathyroid hormone
Adipose tissue
Parathyroid Hormone/pharmacology
030209 endocrinology & metabolism
White adipose tissue
Lipolysis/drug effects
Adipose Tissue/drug effects
Young Adult
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Adipose Tissue
Brown
Receptor
Parathyroid Hormone
Type 1/metabolism
Adipocyte
Internal medicine
Brown adipose tissue
Adipocytes
medicine
Humans
Uncoupling Protein 1
Receptor
Parathyroid Hormone
Type 1
Aged
Chemistry
Thermogenesis/drug effects
Thermogenesis
Uncoupling Protein 1/metabolism
General Medicine
Middle Aged
Thermogenin
Cross-Sectional Studies
medicine.anatomical_structure
Adipose Tissue
Parathyroid Hormone
030220 oncology & carcinogenesis
Female
hormones
hormone substitutes
and hormone antagonists
Adipocytes/drug effects
Zdroj: Breining, P, Pedersen, S B, Kjolby, M, Hansen, J B, Jessen, N & Richelsen, B 2021, ' Parathyroid hormone receptor stimulation induces human adipocyte lipolysis and browning ', European Journal of Endocrinology, vol. 184, no. 5, pp. 687-697 . https://doi.org/10.1530/EJE-20-0713
ISSN: 1479-683X
0804-4643
DOI: 10.1530/eje-20-0713
Popis: Objective Activation of brown adipose tissue is a promising strategy to treat and prevent obesity and obesity-related disorders. Activation of uncoupling protein 1 (UCP1) leads to uncoupled respiration and dissipation of stored energy as heat. Induction of UCP1-rich adipocytes in white adipose tissue, a process known as ‘browning’, serves as an alternative strategy to increase whole body uncoupling capacity. Here, we aim to assess the association between parathyroid hormone (PTH) receptor expression and UCP1 expression in human adipose tissues and to study PTH effects on human white and brown adipocyte lipolysis and UCP1 expression. Design A descriptive study of human neck adipose tissue biopsies substantiated by an interventional study on human neck-derived adipose tissue cell models. Methods Thermogenic markers and PTH receptor gene expression are assessed in human neck adipose tissue biopsies and are related to individual health records. PTH-initiated lipolysis and thermogenic gene induction are assessed in cultured human white and brown adipocyte cell models. PTH receptor involvement is investigated by PTH receptor silencing. Results PTH receptor gene expression correlates with UCP1 gene expression in the deep-neck adipose tissue in humans. In cell models, PTH receptor stimulation increases lipolysis and stimulates gene transcription of multiple thermogenic markers. Silencing of the PTH receptor attenuates the effects of PTH indicating a direct PTH effect via this receptor. Conclusion PTH 1 receptor stimulation by PTH may play a role in human adipose tissue metabolism by affecting lipolysis and thermogenic capacity.
Databáze: OpenAIRE
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