Elemental concentrations in Choroid-RPE and retina of human eyes with age-related macular degeneration
| Autor: | Konerirajapuram Natarajan Sulochana, Muthuvel Bharathselvi, Seeneevasan Aberami, Karunakaran Coral, Sampath Nikhalashree, Jyotirmay Biswas |
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| Rok vydání: | 2019 |
| Předmět: |
inorganic chemicals
0301 basic medicine Chromium Male medicine.medical_specialty genetic structures Retinal Pigment Epithelium Retina Arsenic 03 medical and health sciences Cellular and Molecular Neuroscience chemistry.chemical_compound Macular Degeneration 0302 clinical medicine Nickel Cornea Ophthalmology Metals Heavy medicine Cadaver Humans Aged Aged 80 and over business.industry GCLM Choroid Eye bank Retinal Macular degeneration medicine.disease eye diseases Sensory Systems 030104 developmental biology GCLC medicine.anatomical_structure chemistry Lead 030221 ophthalmology & optometry Female sense organs business Cadmium |
| Zdroj: | Experimental eye research. 186 |
| ISSN: | 1096-0007 |
| Popis: | Heavy metals, metallic and toxic elements are reported to play an essential role in the complex multifactorial pathogenesis of age-related macular degeneration (AMD). This study was aimed to measure the concentrations of these elements in choroid-RPE and retina of human donor eyes with and without age-related macular degeneration associated changes. Human cadaver donor eyeballs were obtained from the CU Shah eye bank, Sankara Nethralaya Eye Hospital, India, after removal of the cornea. 39 control and 51 AMD donor eyes were used in this study. Alabama grading was done on the histopathological sections to identify early and late age-related macular degeneration changes. Concentrations of lead, cadmium, chromium, cobalt, nickel, arsenic and selenium were determined in choroid-RPE and retina using Inductively Coupled Plasma Mass Spectrometer (ICP-MS). Further, gene expression of oxidative stress-related genes, Nrf-2, HO-1, GCLC, GCLM, and detoxification related gene GSTpi was performed. The data were analyzed for statistical significance using Graph Pad® Prism 5 software. Donor eyes with early and late AMD had significantly higher levels of lead, cadmium, chromium, arsenic, and nickel in choroid-RPE and retina compared to the control eyes. Selenium was significantly increased in late AMD compared to control. No significant difference was observed in the levels of cobalt between eyes with and without AMD. Decreased transcript levels of oxidative stress-related genes were observed in the choroid-RPE and retinal tissues. Nrf-2 (p |
| Databáze: | OpenAIRE |
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