Effects of Multiple Injection of Bone Marrow Mononuclear Cells on Spinal Cord Injury of Rats
| Autor: | Chizuka Ide, Kenji Kanekiyo, Masanori Fukushima, Yoshihiro Yamada, Tamami Homma, Masahiro Tamachi, Norihiko Nakano, Yoshihisa Suzuki, Fukuki Saito |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Fourth ventricle Mesenchymal Stem Cell Transplantation Peripheral blood mononuclear cell Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine Cerebrospinal fluid medicine Animals Spinal cord injury Spinal Cord Injuries medicine.diagnostic_test Lumbar puncture business.industry Recovery of Function medicine.disease Surgery Nerve Regeneration Rats Transplantation 030104 developmental biology medicine.anatomical_structure Anesthesia Leukocytes Mononuclear Multiple injection Female Neurology (clinical) Bone marrow business 030217 neurology & neurosurgery |
| Zdroj: | Journal of neurotrauma. 34(21) |
| ISSN: | 1557-9042 |
| Popis: | The effects of multiple injection of bone marrow mononuclear cells (BMNCs) on spinal cord injury (SCI) were compared with those of single injection in rats. BMNCs separated by density-gradient centrifugation from a bone marrow perfusate were injected three times (once weekly) through the cerebrospinal fluid (CSF) via the fourth ventricle, and the locomotor improvement and tissue recovery, including axonal regeneration, were compared with those of single injection. While the single-injection group showed a steep elevation of the Basso-Beattie-Bresnahan (BBB) score 1 week after transplantation, the multiple-injection group maintained a similar steep elevation for 2 weeks after transplantation, and the BBB scores of the multiple-injection group remained thereafter at a level approximately 2-3 points higher than those of the single-injection group until the end of the experiment. There were significant differences between the single- and multiple-injection groups at 3, 4, and 8 weeks after transplantation. The difference in BBB scores at 8 weeks after transplantation suggested that there was a marked difference in the quality of locomotor behaviors between the single-and multiple-injection groups at this stage. An extensive outgrowth of regenerating axons through the astrocyte-devoid areas and a marked reduction of cavity formation were found in both the single- and multiple-injection groups. There were, however, no significant differences in the density of regenerating axons or volumes of cavities between the single- and multiple-injection groups. These results showed that although tissue recoveries were similar between single and multiple injection, the multiple injection of BMNCs was more beneficial for locomotor improvement than single injection for the treatment of SCI. Considering the technically simple and low-cost procedures for the preparation and injection of BMNCs, multiple injection of BMNCs by lumbar puncture has an advantage over single injection on clinical application. |
| Databáze: | OpenAIRE |
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