Pitfalls in the assay of carboxymethylcellulase activity

Autor:	W. A. Lindner, C. Dennison, G. V. Quicke
Rok vydání:	1983
Předmět:	chemistry.chemical_classification biology Trichoderma viride Substrate (chemistry) Bioengineering Cellulase biology.organism_classification Applied Microbiology and Biotechnology Enzyme assay Enzyme Biochemistry chemistry Trichoderma biology.protein Glycoside hydrolase Trichoderma reesei Biotechnology
Zdroj:	Biotechnology and bioengineering. 25(2)
ISSN:	0006-3592
Popis:	A purified endocellulase from Sclerotium rolfsii and a crude cellulase preparation from Trichoderma reesei are used to illustrate several pitfalls associated with the assay of carboxymethylcellulase activity and the subsequent attainment of linear enzyme dilution curves. It is shown that the nature of both the enzymes and the substrate make the assay unsuitable for use in the calculation of enzyme recovery and purity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b9379b12b4d258ce6f090d7b7b8640c2 https://pubmed.ncbi.nlm.nih.gov/18548658 Zobrazit plný text záznamu Plný text