MHC class I and MHC class II DRB gene variability in wild and captive Bengal tigers (Panthera tigris tigris)
| Autor: | Surendra Prakash Goyal, Ina Pokorny, Reeta Sharma, Ralph Tiedemann, Sudanshu Mishra |
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| Rok vydání: | 2010 |
| Předmět: |
Conservation genetics
Felidae Genes MHC Class II Molecular Sequence Data Immunology Genes MHC Class I India Sequence Homology Animals Wild chemical and pharmacologic phenomena Balancing selection Major histocompatibility complex Feces Genetic variation MHC class I Genetics Animals Amino Acid Sequence Tigers Allele Alleles Phylogeny MHC class II Polymorphism Genetic biology Endangered Species fungi Genetic Variation DNA biology.organism_classification Amino Acid Substitution biology.protein Animals Zoo sense organs Sequence Alignment Bengal tiger |
| Zdroj: | Immunogenetics. 62:667-679 |
| ISSN: | 1432-1211 0093-7711 |
| DOI: | 10.1007/s00251-010-0475-7 |
| Popis: | Bengal tigers are highly endangered and knowledge on adaptive genetic variation can be essential for efficient conservation and management. Here we present the first assessment of allelic variation in major histocompatibility complex (MHC) class I and MHC class II DRB genes for wild and captive tigers from India. We amplified, cloned, and sequenced alpha-1 and alpha-2 domain of MHC class I and beta-1 domain of MHC class II DRB genes in 16 tiger specimens of different geographic origin. We detected high variability in peptide-binding sites, presumably resulting from positive selection. Tigers exhibit a low number of MHC DRB alleles, similar to other endangered big cats. Our initial assessment-admittedly with limited geographic coverage and sample size-did not reveal significant differences between captive and wild tigers with regard to MHC variability. In addition, we successfully amplified MHC DRB alleles from scat samples. Our characterization of tiger MHC alleles forms a basis for further in-depth analyses of MHC variability in this illustrative threatened mammal. |
| Databáze: | OpenAIRE |
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