Recognition of prostate-specific antigenic peptide determinants by human CD4 and CD8 T cells
| Autor: | N K Nanda, E E Sercarz, J M Corman |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Adult
CD4-Positive T-Lymphocytes Glutamate Carboxypeptidase II Male Molecular Sequence Data Immunology Epitopes T-Lymphocyte Carboxypeptidases CD8-Positive T-Lymphocytes Biology Epitope Prostate cancer Immune system Antigen HLA-DR4 Antigen medicine Humans Immunology and Allergy Cytotoxic T cell Amino Acid Sequence Antigen-presenting cell Cells Cultured T lymphocyte Prostate-Specific Antigen medicine.disease Prostate-specific antigen Antigens Surface Original Article Epitope Mapping |
| Zdroj: | Clinical and Experimental Immunology. 114:166-172 |
| ISSN: | 1365-2249 0009-9104 |
| Popis: | SUMMARYIt is now becoming accepted that one is not tolerant to all the determinants of self proteins: the T cell repertoire directed to some sequences in self proteins is intact and can be activated. When a self protein is exclusively expressed by tumour cells, the T cell repertoire directed to the particular self antigen can potentially be activated to attack the tumour: this would amount to induction of a beneficial autoimmune response. Prostate cancer offers a unique opportunity for activation of a tumour-specific immune response owing to the exclusive synthesis of prostate-specific antigen (PSA) and prostate-specific membrane antigen (PSM) by prostatic tissue and prostate tumour cells. In this study we examine the CD4 and CD8 T cell repertoires specific for peptides of PSA and PSM in normal human male individuals, using short-term, peptide antigen-driven CD4 and CD8 T cell lines. We show that short-term, CD4 T cell lines derived from six HLA-DR4 individuals showed strong proliferative responses to six of 10 tested peptides of PSA, selected as to contain a DR4 binding motif. Short-term, CD8 T cell lines from three HLA-A1 individuals showed specific cytolytic activity for autologous targets loaded with five of five tested peptides of PSA and PSM, selected to possess an HLA-A1 binding motif. One of the peptides chosen is termed a ‘dual-motif’ peptide, as it encodes determinants for both CD4 and CD8 T cells. These results, indicating the existence of CD4 and CD8 T cells against determinants of the self proteins, PSA and PSM, in healthy male individuals reveal the potential of the T cell repertoire from the typical prostate cancer patient to eradicate prostate tumours upon being appropriately activated. |
| Databáze: | OpenAIRE |
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