Functional and Biochemical Characterization of Alvinella pompejana Cys-Loop Receptor Homologues
| Autor: | Els Pardon, Sarah Debaveye, Marijke Brams, Rouslan G. Efremov, Daniel Bertrand, Mieke Nys, Jan Steyaert, Chris Ulens, Katrien Willegems, Eveline Wijckmans |
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| Přispěvatelé: | Department of Bio-engineering Sciences, Structural Biology Brussels, Faculty of Sciences and Bioengineering Sciences, Zeth, Kornelius |
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Proteome Physiology Taurine Surfactants lcsh:Medicine Ligands Biochemistry Ion Channels Sequence Analysis Protein Medicine and Health Sciences Amino Acids Receptor lcsh:Science Glycine receptor Cysteine Loop Ligand-Gated Ion Channel Receptors gamma-Aminobutyric Acid Crystallography Multidisciplinary biology Organic Compounds Protein Stability Physics Temperature Neurochemistry Neurotransmitters Melting Condensed Matter Physics Ligand (biochemistry) Electrophysiology Chemistry Physical Sciences Crystal Structure Alvinella pompejana Ligand-gated ion channel Sequence Analysis Phase Transitions Research Article Multiple Alignment Calculation Materials Science Detergents Green Fluorescent Proteins Molecular Sequence Data Glycine Biophysics Neurophysiology Sequence alignment Research and Analysis Methods 03 medical and health sciences Computational Techniques Solid State Physics Animals Amino Acid Sequence Molecular Biology Techniques Sequencing Techniques Molecular Biology Materials by Attribute Ion channel Ions Sequence Homology Amino Acid Organic Chemistry lcsh:R Chemical Compounds Biology and Life Sciences Proteins Polychaeta Ligand-Gated Ion Channels Single-Domain Antibodies biology.organism_classification Split-Decomposition Method Protein Subunits 030104 developmental biology Aliphatic Amino Acids lcsh:Q Protein Multimerization Sequence Alignment Neuroscience |
| Zdroj: | PLoS ONE, Vol 11, Iss 3, p e0151183 (2016) PLoS ONE |
| DOI: | 10.1371/journal.pone.0151183 |
| Popis: | Cys-loop receptors are membrane spanning ligand-gated ion channels involved in fast excitatory and inhibitory neurotransmission. Three-dimensional structures of these ion channels, determined by X-ray crystallography or electron microscopy, have revealed valuable information regarding the molecular mechanisms underlying ligand recognition, channel gating and ion conductance. To extend and validate the current insights, we here present promising candidates for further structural studies. We report the biochemical and functional characterization of Cys-loop receptor homologues identified in the proteome of Alvinella pompejana, an extremophilic, polychaete annelid found in hydrothermal vents at the bottom of the Pacific Ocean. Seven homologues were selected, named Alpo1-7. Five of them, Alpo2-6, were unidentified prior to this study. Two-electrode voltage clamp experiments revealed that wild type Alpo5 and Alpo6, both sharing remarkably high sequence identity with human glycine receptor a subunits, are anion-selective channels that can be activated by glycine, GABA and taurine. Furthermore, upon expression in insect cells fluorescence size-exclusion chromatography experiments indicated that four homologues, Alpo1, Alpo4, Alpo6 and Alpo7, can be extracted out of the membrane by a wide variety of detergents while maintaining their oligomeric state. Finally, large-scale purification efforts of Alpo1, Alpo4 and Alpo6 resulted in milligram amounts of biochemically stable and monodisperse protein. Overall, our results establish the evolutionary conservation of glycine receptors in annelids and pave the way for future structural studies. |
| Databáze: | OpenAIRE |
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