The lipoprotein Pal stabilises the bacterial outer membrane during constriction by a mobilisation-and-capture mechanism
| Autor: | Karthik V. Rajasekar, Firdaus Samsudin, Patrice Rassam, Syma Khalid, Christina Redfield, Seán M. Murray, Renata Kaminska, Joanna Szczepaniak, Peter C. Holmes, Patrick George Inns, Colin Kleanthous |
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| Přispěvatelé: | Department of Biochemistry, University of Oxford, University of Oxford, Department of Chemistry, University of Southampton, University of Southampton, LOEWE Center for Synthetic Microbiology (SYNMIKRO), Philipps Universität Marburg = Philipps University of Marburg, European Project: 742555,OMPorg |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Cell division Science General Physics and Astronomy Plasma protein binding Peptidoglycan General Biochemistry Genetics and Molecular Biology Article Cell wall 03 medical and health sciences 0302 clinical medicine Escherichia coli Inner membrane [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology lcsh:Science Barrier function cellular microbiology Multidisciplinary bacterial structural biology Chemistry organic chemicals Escherichia coli Proteins Membrane Proteins General Chemistry humanities Cell biology lipoproteins 030104 developmental biology Membrane Bacterial Outer Membrane Membrane protein bacteria lcsh:Q Periplasmic Proteins Bacterial outer membrane 030217 neurology & neurosurgery Cell Division Bacterial Outer Membrane Proteins |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Nature Communications Nature Communications, 2020, 11 (1), pp.1305. ⟨10.1038/s41467-020-15083-5⟩ |
| ISSN: | 2041-1723 |
| DOI: | 10.1038/s41467-020-15083-5⟩ |
| Popis: | Coordination of outer membrane constriction with septation is critical to faithful division in Gram-negative bacteria and vital to the barrier function of the membrane. This coordination requires the recruitment of the peptidoglycan-binding outer-membrane lipoprotein Pal at division sites by the Tol system. Here, we show that Pal accumulation at Escherichia coli division sites is a consequence of three key functions of the Tol system. First, Tol mobilises Pal molecules in dividing cells, which otherwise diffuse very slowly due to their binding of the cell wall. Second, Tol actively captures mobilised Pal molecules and deposits them at the division septum. Third, the active capture mechanism is analogous to that used by the inner membrane protein TonB to dislodge the plug domains of outer membrane TonB-dependent nutrient transporters. We conclude that outer membrane constriction is coordinated with cell division by active mobilisation-and-capture of Pal at division septa by the Tol system. The lipoprotein Pal participates in the coordination of outer-membrane constriction with septation in Gram-negative bacteria. Here, the authors show that this coordination is mediated by active mobilisation-and-capture of Pal at division septa by the Tol system. |
| Databáze: | OpenAIRE |
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