Polystyrene nanoplastics affect the human ubiquitin structure and ubiquitination in cells: a high-resolution study

Autor: M. della Valle, G. D'Abrosca, M. T. Gentile, L. Russo, C. Isernia, S. Di Gaetano, R. Avolio, R. Castaldo, M. Cocca, G. Gentile, G. Malgieri, M. E. Errico, R. Fattorusso
Přispěvatelé: della Valle, M, D'Abrosca, G, Gentile, Mt, Russo, L, Isernia, C, Di Gaetano, S, Avolio, R, Castaldo, R, Gentile, G, Cocca, M, Malgieri, G, Errico, Me, Fattorusso, R
Rok vydání: 2022
Předmět:
Zdroj: Chemical Science. 13:13563-13573
ISSN: 2041-6539
2041-6520
DOI: 10.1039/d2sc04434j
Popis: Humans are estimated to consume several grams per week of nanoplastics (NPs) through exposure to a variety of contamination sources. Nonetheless, the effects of these polymeric particles on living systems are still mostly unknown. Here, by means of CD, NMR and TEM analyses, we describe at an atomic resolution the interaction of ubiquitin with polystyrene NPs (PS-NPs), showing how a hard protein corona is formed. Moreover, we report that in human HeLa cells exposure to PS-NPs leads to a sensible reduction of ubiquitination. Our study overall indicates that PS-NPs cause significant structural effects on ubiquitin, thereby influencing one of the key metabolic processes at the base of cell viability.
Databáze: OpenAIRE