Nucleation, stabilization, and disassembly of branched actin networks
| Autor: | Fred E. Fregoso, Roberto Dominguez, Alexis Gautreau, Gleb Simanov |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Mechanosensation
Cryoelectron Microscopy Nucleation Arp2/3 complex Motility Actin filament nucleation Saccharomyces cerevisiae macromolecular substances Cell Biology Biology Branching (polymer chemistry) Article Actin-Related Protein 2-3 Complex Actins Actin Cytoskeleton Organelle Biophysics biology.protein Humans biological phenomena cell phenomena and immunity Actin Protein Binding |
| Zdroj: | Trends Cell Biol |
| ISSN: | 0962-8924 |
| DOI: | 10.1016/j.tcb.2021.10.006 |
| Popis: | Arp2/3 complex is an actin filament nucleation and branching machinery conserved in all eukaryotes from yeast to human. Arp2/3 complex branched networks generate pushing forces that drive cellular processes ranging from membrane remodeling to cell and organelle motility. Several molecules regulate these processes by directly inhibiting or activating Arp2/3 complex and by stabilizing or disassembling branched networks. Here, we review recent advances in our understanding of Arp2/3 complex regulation, including high-resolution cryoelectron microscopy (cryo-EM) structures that illuminate the mechanisms of Arp2/3 complex activation and branch formation, and novel cellular pathways of branch formation, stabilization, and debranching. We also identify major gaps in our understanding of Arp2/3 complex inhibition and branch stabilization and disassembly. |
| Databáze: | OpenAIRE |
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