NMR and MS evidences for a random assembled O-specific chain structure in the LPS of the bacterium Xanthomonas campestris pv. Vitians A case of unsystematic biosynthetic polymerization
| Autor: | Anders Broberg, Jens Ø. Duus, Rosa Lanzetta, Cristina De Castro, Michelangelo Parrilli, Bent O. Petersen, Antonio Molinaro |
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| Přispěvatelé: | Molinaro, Antonio, DE CASTRO, Cristina, Lanzetta, Rosa, Parrilli, Michelangelo, BENT O., Petersen, Anders, Broberg, Jens, Duus, C., DE CASTRO, R., Lanzetta, M., Parrilli, B. O., Petersen, A., Broberg, Duus, J. |
| Jazyk: | angličtina |
| Rok vydání: | 2002 |
| Předmět: |
chemistry.chemical_classification
Lipopolysaccharides Models Molecular Magnetic Resonance Spectroscopy biology Phenol O Antigens Nuclear magnetic resonance spectroscopy biology.organism_classification Polysaccharide Xanthomonas campestris Biochemistry Chain structure chemistry.chemical_compound Biosynthesis chemistry Polymerization Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Carbohydrate Conformation Carbohydrate conformation Bacteria |
| Popis: | Xanthomonas campestris pv. vitians is a Gram-negative plant-associated bacterium that acts as causative agent of bacterial leaf spot and headrot in lettuce. The lipopolysaccharide of this bacterium is suspected to be an important molecule for adhesion to and infection of the plants. The lipopolysaccharide has been isolated from the phenol phase and the O-specific chain characterized by compositional analysis, high field NMR and MALDI-TOF MS. It consists of a nonrepetitive branched polysaccharide with a rhamnan backbone to which Fuc3NAc is linked. The NMR and MS approach led to the characterization of the fine structure of the polymer, which is randomly assembled. The rhamnan backbone is built up of beta-Rhap and alpha-Rhap, this last is present in one, two or three adjacent units and branched by an alpha-Fucp3NAc unit. This is a real case of a random constituted O-specific chain, therefore biosynthetic studies towards the comprehension of this irregular biosynthesis are needed. |
| Databáze: | OpenAIRE |
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