Determination of thermo-oxidation products of plant sterols
| Autor: | Anna-Maija Lampi, Vieno Piironen, Laura Juntunen, Jari Toivo |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Rapeseed
Chromatography Gas Clinical Biochemistry 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry chemistry.chemical_compound 0404 agricultural biotechnology Solid phase extraction Chromatography beta-Sitosterol Stigmasterol Autoxidation 010401 analytical chemistry Extraction (chemistry) Temperature Phytosterols Reproducibility of Results 04 agricultural and veterinary sciences Cell Biology General Medicine 040401 food science 6. Clean water 0104 chemical sciences chemistry Gas chromatography Oxidation-Reduction Saponification |
| Zdroj: | University of Helsinki |
| ISSN: | 1570-0232 |
| Popis: | Plant sterols are subjected to oxidation when exposed to air and, especially, when heated at high temperatures. We developed a method to study thermo-oxidation of plant sterols. The method consisted of cold saponification, purification of oxides by solid-phase extraction and gas chromatography analysis. To compensate for losses during the procedure, an internal standard was added before saponification. The method showed good recovery of added cholesterol oxides, separation of plant sterol oxides and reproducibility in detecting thermo-oxidation products of stigmasterol and rapeseed oil. Based on this study, the major products are 7-hydroxy, 5,6-epoxy and 7-keto compounds and oxides are formed faster in bulk stigmasterol than in rapeseed oil. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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