Infrequent CDKN2 (MTS1/p16) gene alterations in human primary breast cancer
| ISSN: | 1532-1827 0007-0920 |
|---|---|
| DOI: | 10.1038/bjc.1995.442 |
| Přístupová URL adresa: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b92743d32616659abd5d578e1c0e0ffb https://doi.org/10.1038/bjc.1995.442 |
| Rights: | OPEN |
| Přírůstkové číslo: | edsair.doi.dedup.....b92743d32616659abd5d578e1c0e0ffb |
| Autor: | J. G. M. Klijn, Nelleke A. Gruis, I. L. Van Staveren, John A. Foekens, Marcel Smid, Emjj Berns |
| Rok vydání: | 1995 |
| Předmět: |
Cancer Research
Cell division Tumor suppressor gene Molecular Sequence Data Mammary gland Breast Neoplasms Biology medicine.disease_cause Exon Cyclin D1 medicine Humans Enzyme Inhibitors skin and connective tissue diseases Protein Kinase Inhibitors Gene Cyclin-Dependent Kinase Inhibitor p16 Cyclin Base Sequence Molecular biology medicine.anatomical_structure Oncology Mutation Cancer research Female Carrier Proteins Carcinogenesis Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| DOI: | 10.1038/bjc.1995.442 |
| Popis: | Changes which lead to excessive cyclin production or to loss of cell cycle inhibition by proteins such as p16/MTS1 may release breast tumour cells from the constraints of cell division. In order to establish the frequency of MTS1/p16 gene alteration and its relation with genetic damage to the p53 and cyclin D1 genes, we have studied these gene abnormalities in 164 human primary breast cancers and in six breast cancer cell lines. Two breast cancer cell lines and one primary tumour showed a homozygous deletion of exon 2 of the MTS1 gene. Using single-strand conformation polymorphism and subsequent sequencing analysis, one tumour showed an alteration at codon 67 (CCC-->CTC; Pro to Leu). Another tumour showed a mutation at codon 98 (without amino acid change) with an additional polymorphism at codon 140. This polymorphism was also found in 13 other tumour samples, but has no effect on (disease-free) survival. From these data we conclude that the occurrence of CDKN2 (p16/MTS1) mutation in primary breast cancer is a rare event and is not likely to be involved in human breast tumour carcinogenesis and progression. Images Figure 1 |
| Databáze: | OpenAIRE |
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