Galectin 1 expression is associated with tumor invasion and metastasis in stage IB to IIA cervical cancer
| Autor: | Young Ae Park, Ha-Jeong Kim, Jung-Joo Choi, Byoung-Gie Kim, Young Jae Cho, Hye-Kyung Jeon, In-Gu Do, Duk-Soo Bae, Jeong-Won Lee, Chel Hun Choi, Tae-Joong Kim, Chang Ohk Sung, Yoo-Young Lee |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty Stromal cell Galectin 1 Angiogenesis Uterine Cervical Neoplasms Adenocarcinoma Biology Pathology and Forensic Medicine Metastasis HeLa Carcinoma Adenosquamous Cell Line Tumor otorhinolaryngologic diseases medicine Humans Neoplasm Invasiveness Neoplastic transformation Neoplasm Staging Cell growth medicine.disease biology.organism_classification stomatognathic diseases Galectin-1 Carcinoma Squamous Cell Female Stromal Cells HeLa Cells |
| Zdroj: | Human Pathology. 44:62-68 |
| ISSN: | 0046-8177 |
| Popis: | Galectin 1 is a 14-kd laminin-binding lectin involved in important biologic mechanisms of tumors, including neoplastic transformation, cell survival, angiogenesis, cell proliferation, and metastasis. In this study, we investigated the role of galectin 1 in cell survival and metastasis in cervical cancer. The expression of galectin 1 was determined in 73 formalin-fixed, paraffin-embedded cervical cancer tissues using an immunohistochemical method and compared with clinicopathologic risk factors for recurrence after surgery. To evaluate the role of galectin 1 in cell proliferation and invasion, we performed proliferation and invasion assays with galectin 1 small interfering RNA (siRNA) using cervical cancer cell lines, including HeLa and SiHa cells. Immunohistochemical analysis revealed that galectin 1 expression was found in most peritumoral stroma samples (72/73; 98.6%). Galectin 1 expression was significantly correlated with the depth of invasion in the cervix (P=.015) and lymph node metastasis (P=.045) on univariate analysis. When progression-free survival of all of the patients studied was analyzed based upon galectin 1 expression, galectin 1 expression was not correlated with progression-free survival (P=.32). Down-regulation of galectin 1 using small interfering RNA resulted in the inhibition of cell growth and proliferation of HeLa and SiHa cells. Moreover, the ability of cells to invade was significantly reduced by galectin 1 small interfering RNA. Our results revealed that high galectin 1 expression in peritumoral stroma was significantly correlated with depth of invasion in cervical lesions and lymph node metastasis of cervical cancer and that galectin 1 may be functionally involved in cell proliferation and invasion. |
| Databáze: | OpenAIRE |
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