Circulating miR-320b and miR-483-5p levels are associated with COVID-19 in-hospital mortality
| Autor: | Angelica Giuliani, Giulia Matacchione, Deborah Ramini, Mirko Di Rosa, Anna Rita Bonfigli, Jacopo Sabbatinelli, Vladia Monsurrò, Rina Recchioni, Fiorella Marcheselli, Francesca Marchegiani, Francesco Piacenza, Maurizio Cardelli, Roberta Galeazzi, Giovanni Pomponio, Alessia Ferrarini, Armando Gabrielli, Silvia Svegliati Baroni, Marco Moretti, Riccardo Sarzani, Piero Giordano, Antonio Cherubini, Andrea Corsonello, Roberto Antonicelli, Antonio Domenico Procopio, Manuela Ferracin, Massimiliano Bonafè, Fabrizia Lattanzio, Fabiola Olivieri |
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| Přispěvatelé: | Giuliani A., Matacchione G., Ramini D., Di Rosa M., Bonfigli A.R., Sabbatinelli J., Monsurro V., Recchioni R., Marcheselli F., Marchegiani F., Piacenza F., Cardelli M., Galeazzi R., Pomponio G., Ferrarini A., Gabrielli A., Svegliati Baroni S., Moretti M., Sarzani R., Giordano P., Cherubini A., Corsonello A., Antonicelli R., Procopio A.D., Ferracin M., Bonafe M., Lattanzio F., Olivieri F. |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
Aging Time Factors Risk Assessment Article Predictive Value of Tests Risk Factors 80 and over Humans Circulating MicroRNA Hospital Mortality RNA-Seq Aged Aged 80 and over COVID-19 MiR-320b MicroRNA Prognosis Up-Regulation Hospitalization MicroRNAs In-hospital mortality Female MiR-483-5p Biomarkers Developmental Biology |
| Zdroj: | Mechanisms of Ageing and Development |
| Popis: | The stratification of mortality risk in COVID-19 patients remains extremely challenging for physicians, especially in older patients. Innovative minimally invasive molecular biomarkers are needed to improve the prediction of mortality risk and better customize patient management. In this study, aimed at identifying circulating miRNAs associated with the risk of COVID-19 in-hospital mortality, we analyzed serum samples of 12 COVID-19 patients by small RNA-seq and validated the findings in an independent cohort of 116 COVID-19 patients by qRT-PCR. Thirty-four significantly deregulated miRNAs, 25 downregulated and 9 upregulated in deceased COVID-19 patients compared to survivors, were identified in the discovery cohort. Based on the highest fold-changes and on the highest expression levels, 5 of these 34 miRNAs were selected for the analysis in the validation cohort. MiR-320b and miR-483-5p were confirmed to be significantly hyper-expressed in deceased patients compared to survived ones. Kaplan-Meier and Cox regression models, adjusted for relevant confounders, confirmed that patients with the 20% highest miR-320b and miR-483-5p serum levels had three-fold increased risk to die during in-hospital stay for COVID-19. In conclusion, high levels of circulating miR-320b and miR-483-5p can be useful as minimally invasive biomarkers to stratify older COVID-19 patients with an increased risk of in-hospital mortality. |
| Databáze: | OpenAIRE |
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