Genetic and molecular features of Su(P), a gene that interacts with ref(2)P in male fertility of Drosophila melanogaster
| Autor: | A. Bichon, N. Boukhatem, P. Gay, P. Dru, H. Terzian, A.-M. Petitjean, F. Lemeunier, D. Contamine |
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| Rok vydání: | 2001 |
| Předmět: |
Male
DNA Complementary Synaptosomal-Associated Protein 25 Sterility Molecular Sequence Data Locus (genetics) Genes Insect Nerve Tissue Proteins Biology P element Animals Genetically Modified Genetics Animals Drosophila Proteins Allele Cloning Molecular Molecular Biology Gene Alleles Base Sequence Chromosome Mapping Membrane Proteins Nuclear Proteins General Medicine biology.organism_classification Null allele Molecular biology DNA-Binding Proteins Drosophila melanogaster Fertility DNA Transposable Elements Insect Proteins RNA Female Drosophila Protein |
| Zdroj: | Molecular genetics and genomics : MGG. 265(2) |
| ISSN: | 1617-4615 |
| Popis: | The ref(2)P gene is involved in the control of sigma rhabdovirus multiplication in Drosophila melanogaster. ref(2)P activity is also necessary for male fertility. However, in one-third of laboratory strains tested, males that lacked ref(2)P activity were fertile. In all such strains studied, the male sterility phenotype was abolished due to the presence of a particular allele at the Su(P) locus, at 73B1–2. These spontaneous suppressor alleles were dominant. We were able to induce dominant suppressor alleles at the Su(P) locus by X-ray mutagenesis and hybrid dysgenesis, suggesting that null alleles of Su(P) confer the dominant suppressor phenotype. The Su(P) gene was cloned by P element tagging. The P element-tagged alleles identified a Su(P) transcript as a 1.4-kb mRNA produced in the soma of both males and females, which is also abundant in ovaries. |
| Databáze: | OpenAIRE |
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