Multiple-pulse damage thresholds of retinal explants in the ns-time regime
| Autor: | Scarlett Lipp, Wilhelm Stork, Philipp Elmlinger, Sebastian Kotzur |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Materials science Laser safety Pulse (signal processing) Retinal Retinal explants Laser 01 natural sciences Atomic and Molecular Physics and Optics Article law.invention 010309 optics 03 medical and health sciences chemistry.chemical_compound chemistry law 0103 physical sciences Fluorescence microscope Irradiation Multiple pulse 030304 developmental biology Biotechnology Biomedical engineering |
| Zdroj: | Biomed Opt Express |
| ISSN: | 2156-7085 |
| Popis: | The data situation of laser-induced damage measurements after multiple-pulse irradiation in the ns-time regime is limited. Since the laser safety standard is based on damage experiments, it is crucial to determine damage thresholds. For a better understanding of the underlying damage mechanism after repetitive irradiation, we generate damage thresholds for pulse sequences up to N = 20 000 with 1.8 ns-pulses using a square-core fiber and a pulsed Nd:YAG laser. Porcine retinal pigment epithelial layers were used as tissue samples, irradiated with six pulse sequences and evaluated for damage by fluorescence microscopy. The damage thresholds decreased from 31.16 µJ for N = 1 to 11.56 µJ for N = 20 000. The reduction indicates photo-chemical damage mechanisms after reaching a critical energy dose. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|