Polyelectrolyte precipitation of proteins: II. Models of the particle size distributions

Autor:	Charles E. Glatz, Rod R. Fisher
Rok vydání:	1988
Předmět:	chemistry.chemical_classification Mineralogy Thermodynamics Bioengineering Polymer Kinetic energy Applied Microbiology and Biotechnology Polyelectrolyte Condensed Matter::Soft Condensed Matter Shear rate chemistry Shear (geology) Breakage Egg White Proteins Particle size Biotechnology
Zdroj:	Biotechnology and Bioengineering. 32:786-796
ISSN:	1097-0290 0006-3592
DOI:	10.1002/bit.260320610
Popis:	A population-balance model has been used to characterize continuous polyelectrolyte precipitation of egg white proteins. We have modeled the particle size distributions of aggregates formed under a range of mixing conditions. The models, accounting for aggregate growth (by both shear-driven and Brownian-like collisions), breakage (by hydrodynamic shear or aggregate-aggregate collisions), and birth (by the breakage of large aggregates), fit the data well. The kinetic constants show dependencies on shear rate and residence time that have not been previously theoretically predicted; these dependencies are due in part to aging effects on the aggregate. The model constants show a dominance of growth over breakage, supporting qualitative interpretations of the particle size distributions. A mechanism for growth-rate enhancement, caused by polymer extensions from the particle surfaces, produced improved model performance. A collisional breakage mechanism is supported.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b91f54a84a0d2dfaabe6e42f1443452a https://doi.org/10.1002/bit.260320610 Zobrazit plný text záznamu Plný text