Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels
| Autor: | Amrina Shafi, Insha Zahoor, Mudasir A. Mir |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
Shoot Induction
chemistry.chemical_classification Antioxidant Arabidopsis thaliana biology Chemistry medicine.medical_treatment Regeneration (biology) fungi Callus Culture food and beverages biology.organism_classification In vitro Enzyme Biochemistry Botany medicine Suspension Culture Suspension (vehicle) Biotechnology |
| Zdroj: | International Journal of Trend in Scientific Research and Development. 2:37-45 |
| ISSN: | 2456-6470 |
| DOI: | 10.31142/ijtsrd5843 |
| Popis: | Arabidopsis tissue culture is valuable for any laboratory working on this model plant. Tissue culture methodology facilitates the production of a large number of plants that are genetically identical over a relatively short growth period. Currently this in vitro regeneration system is a good system to study the mechanism by which plants show regenerative plasticity. Plant regeneration is a key technology for successful stable plant transformation, while cell suspension cultures can be exploited for metabolite profiling, kinetic study and mining. In this paper we report methods for the successful and highly efficient in vitro regeneration of plants and production of stable cell suspension lines from cotyledons of Arabidopsis thaliana. It is an easy and reproducible method of regenerating Arabidopsis plants from callus culture. A combination of 6 benzylaminopurine BAP and a naphthalene acetic acid NAA in a Murashige and Skoogs MS based medium gives a high percentage of shoot formation. Further cell suspension culture were used to study the growth kinetics and also for checking level of antioxidant enzymes at different stages of culture. An expression analysis of antioxidant genes such as superoxide dismutase SOD and ascorbate peroxidase APX was also done at callus, shoot and root regeneration stage. It was found that levels and activity of these antioxidant enzymes were higher at regeneration stage, indicating antioxidant enzyme role in plant morphogenesis. Here we describe a standard protocol for regenerating Arabidopsis plants in tissue culture, and for preparing and observing samples using steriosome and bright field microscopy to study different stages of regeneration. Amrina Shafi | Insha Zahoor | Mudasir A Mir "Invitro Regeneration of Arabidopsis Thaliana from Suspension and Invitro Cultures and Analysis of Regeneration and Antioxidant Enzyme Levels" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-1 , December 2017, URL: https://www.ijtsrd.com/papers/ijtsrd5843.pdf |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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