Production of Monoclonal Antibodies AgainstNosema bombycisand their Utility for Detection of Pebrine Infection inBombyx moriL
| Autor: | Satish K. Gupta, R. K. Datta, B. Nataraju, Debjani Ghosh, M. Shamim, M. Baig |
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| Rok vydání: | 1997 |
| Předmět: |
Spores
Immunology Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Sensitivity and Specificity Cross-reactivity Microbiology Mice Nosema Pébrine Microsporidiosis Hemolymph Tumor Cells Cultured medicine Animals Pharmacology Latex beads Mice Inbred BALB C Hybridomas fungi Antibodies Monoclonal Bombyx medicine.disease biology.organism_classification Molecular biology Latex fixation test Spore Agglutination (biology) Latex Fixation Tests |
| Zdroj: | Journal of Immunoassay. 18:357-370 |
| ISSN: | 0197-1522 |
| DOI: | 10.1080/01971529708005827 |
| Popis: | Latex agglutination assay based on monoclonal antibodies (MCAs) described in this communication may be useful for detection of Pebrine infection in silkworm. Four murine MCAs were produced against Nosema bombycis spore. In ELISA all 4 MCAs (IgM isotype) reacted with alkali treated Nosema spores and to variable extent with acetone precipitated surface protein. However, MA-310 and MA-542 showed a low degree of cross reactivity with BmNPV. In contrast, MA-503 and MA-515 were devoid of reactivity with BmNPV, B. thuringiensis, S. marcescens, Azotobactor, Rhizobium and normal hemolymph protein in ELISA. Latex beads sensitized with a combination of MA-503 and MA-515 (50 micrograms each per ml of 0.4% latex beads) could detect 1 x 10(5) Nosema spores per test. Sensitization of the latex beads with the cocktail of these two MCAs through protein-A bridge further led to a 10-fold increase in the sensitivity (1 x 10(4) spores/test) of the assay. No agglutination was observed in presence of BmNPV, Rhizobium, Azotobactor, E. coli, B. thuringiensis, S. marcescens and normal hemolymph protein indicating the specificity of the test. The results obtained by latex agglutination assay on hemolymph samples of infected as well as normal larvae collected from field, II instar larvae infected in the laboratory and from infected mother moth revealed 100% correlation with results by microscopic examination. |
| Databáze: | OpenAIRE |
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