Tryptophan Supplementation Increases the Production of Microbial-Derived AhR Agonists in an in Vitro Simulator of Intestinal Microbial Ecosystem
| Autor: | Jonna EB Koper, Antonio Dario Troise, Linda MP Loonen, Paola Vitaglione, Edoardo Capuano, Vincenzo Fogliano, Jerry M Wells |
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| Přispěvatelé: | Koper, J. E. B., Troise, A. D., Loonen, L. M. P., Vitaglione, P., Capuano, E., Fogliano, V., Wells, J. M. |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
tryptophan metabolites
SHIME aryl hydrocarbon receptor Microbiota Tryptophan General Chemistry respiratory system respiratory tract diseases Gastrointestinal Microbiome Food Quality and Design Receptors Aryl Hydrocarbon tryptophan metabolite WIAS microbiota tryptophan Host-Microbe Interactomics General Agricultural and Biological Sciences VLAG Dietary Supplement Human |
| Zdroj: | Journal of Agricultural and Food Chemistry 70 (2022) 13 Journal of Agricultural and Food Chemistry, 70(13), 3958-3968 |
| ISSN: | 0021-8561 |
| Popis: | The aryl hydrocarbon receptor (AhR) plays an important role in intestinal homeostasis, and some microbial metabolites of tryptophan are known AhR agonists. In this study, we assessed the impact of tryptophan supplementation on the formation of tryptophan metabolites, AhR activation, and microbiota composition in the simulator of the human intestinal microbial ecosystem (SHIME). AhR activation, microbial composition, and tryptophan metabolites were compared during high tryptophan supplementation (4 g/L tryptophan), control, and wash-out periods. During tryptophan supplementation, the concentration of several tryptophan metabolites was increased compared to the control and wash-out period, but AhR activation by fermenter supernatant was significantly decreased. This was due to the higher levels of tryptophan, which was found to be an antagonist of AhR signaling. Tryptophan supplementation induced most microbial changes in the transverse colon including increased relative abundance of lactobacillus. We conclude that tryptophan supplementation leads to increased formation of AhR agonists in the colon. |
| Databáze: | OpenAIRE |
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