Selective chemical autoligation on a double-stranded DNA template
| Autor: | Robert L. Letsinger, Mathias Kurt Herrlein |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Base Sequence
Poly T Pyrimidine Oligonucleotide Base pair Stereochemistry Molecular Sequence Data Nucleic Acid Heteroduplexes DNA Templates Genetic Thionucleotides Biology Nucleic Acid Denaturation chemistry.chemical_compound Nucleic acid thermodynamics Oligodeoxyribonucleotides chemistry Biochemistry Polynucleotide Nucleic Acid Renaturation Genetics Nucleic acid structure |
| Zdroj: | Nucleic Acids Research. 22:5076-5078 |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/22.23.5076 |
| Popis: | We show that a double-stranded DNA segment serves as an effective template for spontaneously coupling short pyrimidine oligonucleotides containing terminal -P(O)(O-)S- and BrCH2C(O)NH- groups. The efficiency of this autoligation depends markedly on proper base-pairing between the probe oligomers and the double-stranded target. This chemistry should be useful in designing highly selective probes for double-stranded polynucleotide segments. |
| Databáze: | OpenAIRE |
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