Extra-Domain-A Fibronectin: A New Marker of Fibrosis in Cutaneous Graft-Versus-Host Disease
| Autor: | Rob Fijnheer, Marijke R. Canninga-van Dijk, Leo F. Verdonck, Jan Aten, Hanneke M. van der Straaten, Domenico Castigliego, H.P. Eric Borst |
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| Přispěvatelé: | AII - Amsterdam institute for Infection and Immunity, Pathology |
| Rok vydání: | 2004 |
| Předmět: |
Adult
Genetic Markers Male Pathology medicine.medical_specialty Graft vs Host Disease Dermatology Biochemistry Extracellular matrix fibronectin Fibrosis graft-versus-host disease medicine Humans RNA Messenger Coloring Agents Molecular Biology Skin Staining and Labeling biology Reverse Transcriptase Polymerase Chain Reaction Cell Biology Middle Aged medicine.disease Immunohistochemistry allogeneic transplantation Fibronectins Protein Structure Tertiary Fibronectin Transplantation surgical procedures operative Real-time polymerase chain reaction Graft-versus-host disease biology.protein Female Stem cell |
| Zdroj: | Journal of investigative dermatology, 123(6), 1057-1062. Nature Publishing Group |
| ISSN: | 0022-202X |
| Popis: | One of the major complications that limit the success of allogeneic stem cell transplantation is graft-versus-host disease (GVHD). The major target organ in GVHD is the skin. Cutaneous GVHD can eventually lead to fibrosis of the skin. Fibronectin mediates a variety of cellular interactions with the extracellular matrix. The molecular and functional diversity of fibronectin (FN) arises from alternative splicing of pre-mRNA. In normal circumstances endothelial cells and fibroblasts synthesize FN without the ED-A domain. In tissue repair and pathologic circumstances such as fibrosis, the ED-A domain is expressed. We hypothesize that expression of ED-A FN is upregulated in patients with cutaneous GVHD. In frozen skin biopsies the expression of ED-A FN was measured at the protein level by immunohistochemistry and at the mRNA level by quantitative real-time PCR (qPCR). In normal control skin, immunohistochemistry showed slight deposits of ED-A FN just under the basal layer. The expression of ED-A FN significantly increased in acute cutaneous GVHD (p |
| Databáze: | OpenAIRE |
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