Intraflagellar transport delivers tubulin isotypes to sensory cilium middle and distal segments

Autor: Gul Civelekoglu-Scholey, Melanie Thein, Jonathan M. Scholey, Ingrid Brust-Mascher, Yun Lu, Shai Shaham, Bram Prevo, Limin Hao, Seyda Acar
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Axoneme
Kidney Disease
Time Factors
Mechanotransduction
Microtubule-associated protein
Sensation
macromolecular substances
Flagellum
Medical and Health Sciences
Mechanotransduction
Cellular
Microtubules
Models
Biological
Fluorescence
Article
03 medical and health sciences
0302 clinical medicine
Polycystic Kidney Disease
Models
Intraflagellar transport
Microtubule
Tubulin
Animals
Cilia
Caenorhabditis elegans
Caenorhabditis elegans Proteins
030304 developmental biology
Microscopy
0303 health sciences
biology
Cilium
Fluorescence recovery after photobleaching
Cell Biology
Biological Sciences
Biological
Cell biology
Protein Transport
Microscopy
Fluorescence
Flagella
Mutation
biology.protein
Cellular
sense organs
Microtubule-Associated Proteins
030217 neurology & neurosurgery
Developmental Biology
Fluorescence Recovery After Photobleaching
Zdroj: Nature cell biology
Nature cell biology, vol 13, iss 7
ISSN: 1476-4679
1465-7392
Popis: Sensory cilia are assembled and maintained by kinesin-2-dependent intraflagellar transport (IFT). We investigated whether two Caenorhabditis elegans α- and β-tubulin isotypes, identified through mutants that lack their cilium distal segments, are delivered to their assembly sites by IFT. Mutations in conserved residues in both tubulins destabilize distal singlet microtubules. One isotype, TBB-4, assembles into microtubules at the tips of the axoneme core and distal segments, where the microtubule tip tracker EB1 is found, and localizes all along the cilium, whereas the other, TBA-5, concentrates in distal singlets. IFT assays, fluorescence recovery after photobleaching analysis and modelling indicate that the continual transport of sub-stoichiometric numbers of these tubulin subunits by the IFT machinery can maintain sensory cilia at their steady-state length.
Databáze: OpenAIRE
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